اثر 8 هفته تمرین شنا و تزریق سلول بنیادی بر برخی عوامل مؤثر در مسیر آنژیوژنز موش‌های مدل نارسایی زودرس تخمدان

نوع مقاله : اصیل پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

2 دانشیار گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

3 استادیار گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

4 استادیار گروه علوم و فناوری‌های زیستی، دانشکده علوم پزشکی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

10.22038/ijogi.2023.74197.5815

چکیده

مقدمه: ناباروری زنان، یک وضعیت پزشکی جهانی است که می ­تواند ناشی از اختلالات مختلف دستگاه تناسلی از جمله نارسایی زودرس تخمدان باشد. مطالعه حاضر با هدف بررسی اثر 8 هفته تمرین شنا و تزریق سلول بنیادی بر ژن ­های VEGF، TGF و HGF بافت تخمدان موش­ های مدل نارسایی زودرس تخمدان انجام شد.
روش‌کار: در این تحقیق تجربی، تعداد 30 سر موش صحرایی ماده 8-6 هفته­ای انتخاب شدند. به‌منظور ایجاد مدل نارسایی زودرس تخمدان، از داروی سیکلوفسفامید و بیوسولفان به ­ترتیب به میزان ۱۰۰ و ۵۰ میلی­گرم بر کیلوگرم، به­ صورت تزریق درون صفاقی استفاده شد. پس از القاء مدل، موش­ ها به­ صورت تصادفی به 6 گروه (5 سر در هر گروه) شامل: کنترل سالم، بیمار+ شم، بیمار+ سالین، بیمار+ سلول، بیمار+ ورزش و بیمار+ سلول+ ورزش تقسیم شدند. رت­ های گروه تمرین، در طی 8 هفته به شنا پرداختند. گروه­ های سلول، 2 هفته بعد از ایجاد مدل سلول­ های بنیادی به­ میزان یک میلیون سلول برای هر موش در تخمدان پیوند زده شد. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS (نسخه 22) و آزمون‌های آنالیز واریانس یک ­طرفه و تعقیبی توکی انجام شد. میزان p کمتر یا مساوی 05/0 معنی‌دار در نظر گرفته شد.
یافته­ها: القاء نارسایی زودرس تخمدان منجر به افزایش معنی­دار ژن های VEGF، TGF و HGF بافت تخمدان رت ­ها نسبت به گروه کنترل- سالم شد (05/0≥p) که گروه­ های تمرین، سلول و تمرین+ سلول، بیان این ژن ­ها را نسبت به گروه بیمار و بیمار سالین کاهش دادند (05/0≥p).
نتیجه ­گیری: اثر هم­ افزایی تمرین هوازی و سلول بنیادی مزانشیمی مشتق از مغز استخوان باعث بهبود فاکتورهای رگ­زایی رت ­های مدل تجربی نارسایی زودرس تخمدان می‌شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of 8 weeks of swimming training and stem cell injection on some effective factors in the angiogenesis pathway of premature ovarian failure model rats

نویسندگان [English]

  • Ghoncheh Salehi 1
  • Parvin Farzanegi 2
  • Abdolreza Jafari 3
  • Farzane Farokhi 4
1 PhD student in Exercise Physiology, Department of Exercise Physiology, School of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.
2 Associate Professor, Department of Exercise Physiology, School of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.
3 Assistant Professor, Department of Exercise Physiology, School of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.
4 Assistant Professor, Department of Biological Sciences and Technologies, School of Medical Sciences, Sari Branch, Islamic Azad University, Sari, Iran.
چکیده [English]

Introduction: Female infertility is a universal medical condition that can be caused by various disorders of the reproductive system, including premature ovarian failure. The present study was performed with aim to investigate the effect of 8 weeks of swimming training and stem cell injection on VEGF, TGF and HGF genes in the ovarian tissue of premature ovarian failure model rats.
Methods: In this experimental research, 30 female rats 6- to 8-week-old were selected. In order to create a model of premature ovarian failure, cyclophosphamide and biosulfan were used in the amount of 100 and 50 mg/kg, respectively, as an intraperitoneal injection. After model induction, rats were randomly divided into 6 groups (5 heads in each group) including: 1) healthy control, 2) patient+ sham, 3) patient+ saline, 4) patient+ cell, 5) patient +exercise and 6) patient+ cell+ exercise. The rats of the training group swam for 8 weeks. Cell groups, 2 weeks after creating the model stem cells were transplanted in the ovary at the rate of one million cells for each mouse. Data analysis was done using SPSS statistical software (version 22) and one-way ANOVA and Tukey's post hoc tests. P≤0.05 was considered significant.
Results: Induction of premature ovarian failure led to a significant increase in VEGF, TGF and HGF genes in the ovarian tissue of rats compared to the control-healthy group (P≤0.05) that the exercise, cell and exercise+ cell groups decreased the expression of these genes compared to the patient and patient-saline groups (P≤0.05).
Conclusion: The synergistic effect of aerobic exercise and bone marrow-derived mesenchymal stem cells improve angiogenic factors in experimental premature ovarian failure model rats.

کلیدواژه‌ها [English]

  • Mesenchymal stem cells
  • Premature ovarian failure
  • Swimming training

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مجله زنان، مامایی و نازایی ایران
دوره 26، شماره 10
دی 1402
صفحه 43-57

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