مقایسه تأثیر والپروات سدیم و سیترونلول بر غلظت استریول، گنادوتروپین جفتی و آلفافیتوپروتئین در سرم مادر و جنین و مایع آمنیوتیک در موش صحرایی

نوع مقاله : اصیل پژوهشی

نویسندگان

1 دانشیار گروه آناتومی مقایسه‌ای، مرکز تحقیقات بیماری‌های غیرواگیر، دانشکده پزشکی، دانشگاه علوم پزشکی جهرم، جهرم، ایران.

2 پزشک عمومی، کمیته تحقیقات دانشجویی، دانشکده پزشکی، دانشگاه علوم پزشکی جهرم، جهرم ایران.

3 استادیار گروه علوم تشریحی، دانشکده پزشکی، دانشگاه علوم پزشکی جهرم، جهرم، ایران.

4 استادیار بافت‌شناسی مقایسه‌ای، مرکز تحقیقات بیماری‌های غیرواگیر، دانشکده پزشکی، دانشگاه علوم پزشکی جهرم، جهرم، ایران.

چکیده

مقدمه: مصرف بسیاری از داروهای ضدصرع مانند والپروات سدیم در دوران بارداری، دارای اثرات مخرب و تراتوژنیک می­ باشد. بنابراین مطالعه حاضر با هدف مقایسه تأثیر سدیم والپروات و داروی کم‌خطرتر سیترونلول بر سطح متغیرهای سه­ گانه تشخیص ناهنجاری­ های سیستم عصبی در مادر و جنین انجام شد.
روش‌کار: در این مطالعه تجربی که در سال 1398 انجام شد، 18 سر موش صحرایی ماده بالغ از نژاد ویستار (200-220 گرم، 12-10 هفته) به 3 گروه 6تایی شامل کنترل، تجربی 1 (دریافت کننده والپروات سدیم/ 400 میلی­گرم بر کیلوگرم) و تجربی 2 (دریافت کننده سیترونلول/ 400 میلی­ گرم بر کیلوگرم) تقسیم شدند و پس از قرار گرفتن در کنار موش نر و تأیید حاملگی (پلاک واژنی و واژینال اسمیر)، والپروات سدیم و سیترونلول را در روزهای 7، 8، 9 و 10 بارداری به‌صورت داخل صفاقی دریافت نمودند. در روز 18 بارداری، نمونه ­برداری از سرم مادر، سرم جنین و مایع آمنیوتیک انجام گرفت و میزان استریول آزاد، هورمون گنادوتروپین جفتی و آلفافیتوپروتئین با روش الایزا سنجیده شد. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS (نسخه 21) و آزمون آماری آنووا و تست دانکن انجام گرفت. میزان p کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته­ ها: میانگین غلظت استریول، گنادوتروپین جفتی و آلفافیتوپروتئین در سرم مادر، سرم جنین و مایع آمنیوتیک در گروه سیترونلول تفاوت معنی­ داری با گروه کنترل نشان نداد (05/0p>)، اما در گروه والپروات سدیم کاهش معنی­ دار سطح استریول و گنادوتروپین جفتی و افزایش معنی­ دار آلفافیتوپروتئین در سرم مادر، سرم جنین و مایع آمنیوتیک نسبت به گروه­ های کنترل و تجربی 2 مشاهده شد (05/0p<).
نتیجه ­گیری: سیترونلول به‌عنوان یک داروی ضدصرع با عوارض جانبی کمتر و ایمنی بیشتر می ­تواند در دوران بارداری مورد استفاده قرار گیرد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of sodium valproate and citronellol on the concentration of estriol, placental gonadotropin and alpha phytoprotein in maternal and fetal serum and amniotic fluid in rats

نویسندگان [English]

  • Mohammad Pourahmadi 1
  • Sara Bagheri 2
  • Marzieh Rahimipour 3
  • Hossein Kargar Jahromi 4
1 Associate Professor, Department of Comparative Anatomy, Research Center for Non-Communicable Disease, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran.
2 General Physician, Student Research Committee, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran.
3 Assistant Professor, Department of Anatomical Sciences, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran.
4 Assistant Professor, Department of Comparative Histology, Research Center for Non-Communicable Disease, Faculty of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran.
چکیده [English]

Introduction: Taking many antiepileptic drugs such as sodium valproate during pregnancy has harmful and teratogenic effects. Therefore, the present study was performed with aim to compare the effect of sodium valproate and the less dangerous drug citronellol on the level of three variables for the diagnosis of nervous system abnormalities in mother and fetus.
Methods: In this experimental study, which was performed in 2019, eighteen adult female Wistar rats (200-220 g, 10 to 12 weeks) were divided into 3 groups of 6 including control, experimental 1 (receiving sodium valproate, 400 mg/kg) and experimental 2 (receiving citronellol, 400 mg/kg). Female rats were mated with male rats. After confirming pregnancy (vaginal plaque and vaginal smear), they intraperitoneally received sodium valproate and citronellol intraperitoneally on days 7, 8, 9 and 10 of pregnancy. On the 18th day of pregnancy, maternal serum, fetal serum, and amniotic fluid were sampled and free estriol, chorionic gonadotropin, and alpha phytoprotein were measured by ELISA method. Data analysis was done using SPSS statistical software (version 21) and ANOVA and Duncan's test. A p value of less than 0.05 was considered significant.
Results: The mean concentration of estriol, chorionic gonadotropin and alpha phytoprotein in maternal serum, fetal serum and amniotic fluid in citronellol group did not show any significant difference compared to the control group (p>0.05). However, in sodium valproate group, a significant decrease in the level of estriol and placental gonadotropin and a significant increase in alpha phytoprotein in maternal serum, fetal serum, and amniotic fluid were observed compared to the control and experimental groups 2 (p<0.05).
Conclusion: Citronellol as an antiepileptic drug with less side effects and greater safety can be used during pregnancy.

کلیدواژه‌ها [English]

  • Alpha phytoprotein
  • Citronellol
  • Estriol
  • Placental gonadotropin
  • Rat

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مجله زنان، مامایی و نازایی ایران
دوره 26، شماره 8
آبان 1402
صفحه 10-18

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