بررسی ارتباط چندشکلی G>A rs17144343 در ژن LncRNA HULC با استعداد بروز سقط خودبه‌خودی مکرر در زنان آذربایجان‌شرقی، ایران

نوع مقاله : اصیل پژوهشی

نویسندگان

1 کارشناس ارشد زیست‌شناسی سلولی و مولکولی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز، ایران.

2 استادیار گروه زیست‌شناسی سلولی و مولکولی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز، ایران.

3 استادیار ژنتیک پزشکی، آزمایشگاه ژنتیک پزشکی، مرکز درمان ناباروری جهاد دانشگاهی، تبریز، ایران.

4 دانشیار گروه زیست‌شناسی سلولی و مولکولی، دانشکده علوم پایه، دانشگاه شهید مدنی آذربایجان، تبریز، ایران.

چکیده

مقدمه: زنان با بیش از 2 بار تجربه­ سقط جنین، مبتلا به سقط خودبه ­خودی مکرر هستند که حدود 4/1-8/0% زوجین را دربرمی­ گیرند. ژن HULC به‌عنوان یک RNA غیررمزگذار بسیار مهم در سرطان کبد شناسایی شده و بعدها ارتباط آن با برخی از بیماری­ ها شامل سقط جنین مورد توجه قرار گرفت. مطالعه حاضر با هدف بررسی ارتباط چندشکلی تک ­نوکلئوتیدی G>A rs17144343 در ژن HULC با استعداد سقط‌های خودبه­ خودی مکرر در جمعیت زنان آذربایجان­ شرقی انجام شد.
روش‌کار: در این مطالعه­ مورد- شاهدی که در سال­­ های ۹۹-1398 انجام گرفت، DNA ژنومی از خون محیطی 150 فرد بیمار و 150 فرد سالم به روش رسوب نمکی استخراج شد و ژنوتیپ افراد با استفاده از Tetra-ARMS-PCR تعیین شد. داده­ های ژنوتیپی با استفاده از بسته نرم­ افزاری آنلاین جاواستات و SPSS  (نسخه 23) مورد تجزیه و تحلیل آماری قرار گرفت.
یافته ­ها: توزیع ژنوتیپی rs17144343 برای ژنوتیپ­ های GA  وGG  به‌ترتیب در گروه بیماران 7/24% و 3/75% و در گروه کنترل 2% و 98% به‌دست آمد. همچنین فراوانی آللA  در افراد مبتلا 4/12% و در گروه شاهد 1% بود. میان استعداد بروز سقط مکرر و چندشکلی G>A rs17144343 ژن HULC ارتباط معنی‌داری وجود داشت (001/0>p). همچنین در بررسی ارتباط این چندشکلی با برخی ویژگی‌های بالینی و پاتولوژیک، ارتباط معنی‌داری میان ژنوتیپ‌های این لکوس و رابطه خویشاوندی والدین زوجین وجود داشت (003/0=p).
نتیجه­ گیری: میان چندشکلیrs17144343  ژن HULC و استعداد ابتلاء به سقط خودبه­ خودی مکرر ارتباط معنی‌داری مشاهده شد. با این ­وجود، مطالعات بر روی نمونه جمعیتی بزرگ‌تر می ­تواند در تأیید بیشتر این یافته مؤثر باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Association of LncRNAHULC Gene rs17144343 G>APolymorphism with Susceptibility to Recurrent Spontaneous Miscarriage in Women of Eastern Azerbaijan, Iran

نویسندگان [English]

  • Rezvan Hatami 1
  • Solmaz Moniri Javadhesari 2
  • Jafar Mohseni 3
  • Nasser Pouladi 4
1 M.Sc. of Cellular and Molecular Biology, School of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.
2 Assistant Professor, Department of Cellular and Molecular Biology, School of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.
3 Assistant Professor, Department of Medical Genetics, Infertility treatment center, Tabriz, Iran.
4 Associate professor, Department of Cellular and Molecular Biology, School of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.
چکیده [English]

Introduction: Women who experience more than two miscarriages suffer from recurrent pregnancy loss(RPL) which involves about 0.8% to 1.4% of couples. The lncRNA HULC gene was first discovered for its significant regulatory function in Hepatocellular carcinoma and then was considered in other diseases including RPL. The present study was performed with aim to investigate the association of G>A single nucleotide polymorphism of rs17144343 in HULC gene with susceptibility to recurrent miscarriages in women population of Eastern Azerbaijan of Iran.
Methods: In this case-control study which was performed in 2019-2020, genomic DNAs of 150 patients and 150 healthy individuals were extracted from the peripheral blood samples by salting-out method. Then, genotyping of rs17144343 locus was determined by TETRA-ARMS-PCR assay. Finally, the genotyping data were statistically analyzed using the software package javastat online statistics and SPSS (version 23).
Results: The genotypic distribution of rs17144343 for GA and GG genotypes were respectively 24.7% and 75.3% for cases and 2% and 98% for the control group. The frequency of allele A in controls was 1% and in case individuals was 12.4%. There was a significant relationship between the risk of recurrent pregnancy loss and rs17144343 polymorphism of HULC gene (P<0.001). Also, by considering some clinical and pathological characteristics, it was revealed that there is a significant relationship between genotypes and the familial relationship between the couple's parents (P=0.003).
Conclusion: The results obtained in his study demonstrated that G>A polymorphism of rs17144343 in HULC gene has a significant role in susceptibility to recurrent pregnancy loss, but larger sample studies are required to further verify this finding.

کلیدواژه‌ها [English]

  • LncRNAHULC gene
  • Recurrent pregnancy loss
  • rs17144343 Single-nucleotide polymorphism

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مجله زنان، مامایی و نازایی ایران
دوره 26، شماره 1
فروردین 1402
صفحه 63-74

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