تأثیر تمرینات شنا بر مقاومت به انسولین و بیان ژن‌های ادیپونکتین و APPL-1 کبد به‌دنبال القاء سندرم تخمدان پلی‌کیستیک موش‌های صحرایی

نوع مقاله : اصیل پژوهشی

نویسندگان

1 دانشجوی دکتری تربیت بدنی و علوم ورزشی، دانشکده ادبیات، علوم انسانی و اجتماعی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

2 دانشیار گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات، علوم انسانی و اجتماعی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.

3 استادیار گروه زنان و مامایی، دانشکده پزشکی، دانشگاه علوم پزشکی آزاد اسلامی، تهران، ایران.

چکیده

مقدمه: سندرم تخمدان پلی کیستیک (PCOS) یک اختلال اندوکرین شایع در زنان است که با مقاومت به انسولین (IR) ارتباط دارد. فاکتورهایی چون ادیپونکتین و APPL-1 در سیگنالینگ IR نقش دارند که تحت تأثیر ورزش قرار می­ گیرند. مطالعه حاضر هدف تأثیر تمرینات شنا بر مقاومت به انسولین و بیان ژن­ های ادیپونکتین و APPL-1 کبد به‌دنبال القاء سندرم تخمدان پلی ­کیستیک موش­ های صحرایی انجام شد.
روش‌کار: این مطالعه نیمه ­تجربی در سال 1400 36 سر موش صحرایی ماده بالغ نژاد ویستار انجام شد. موش ­ها در 6 گروه قرار گرفتند. تمرین شنا در گروه مداخله تمرینی (6 و 8 هفته/ 5 روز در هفته، هر جلسه 60 دقیقه و تغییر سرعت جریان آب از 715 لیتر در دقیقه) انجام ­شد. 24 ساعت بعد از قربانی شدن موش ­ها، میزان IR، بیان ژن­ های ادیپونکتین و APPL1 از بافت کبد اندازه‌گیری شد. تجزیه و تحلیل داده­ ها با استفاده از نرم‌افزار SPSS (نسخه22) و آزمون تحلیل واریانس دوراهه انجام شد. میزان p کمتر از 05/0 معنی­ دار در نظر گرفته شد.
یافته­ ها: اجرای 6 هفته تمرین بر سطوح سرمی IR (001/0=p) و بیان ژن ادیپونکتین (004/0=p) اثر معناداری داشت، در حالی ­که میزان بیان ژن APPL-1 (433/0=p) تأثیر معناداری نداشت. همچنین 8 هفته تمرین بر میزان IR (001/0=p) و بیان ژن ­های APPL-1 (001/0=p) و ادیپونکتین (001/0=p) تأثیر معناداری داشت.
نتیجه ­گیری: تمرینات شنا می ­تواند منجر به تغییرات معنی ­دار در سطوح سرمی IR، بیان ژن­ های ادیپونکتین و APPL1 شود. به‌نظر می­ رسد 8 هفته تمرین شنا با بهبود بیشتر در مسیر متابولیکی می ­تواند اثرات مثبتی در بیماران PCOS داشته باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Swimming Training on the Insulin Resistance and Expression of Adiponectin and APPL-1 Liver Genes Following Induced Polycystic Ovary Syndrome in Rats

نویسندگان [English]

  • Maryam Khalilinejad 1
  • Mandana Gholami 2
  • Fereshteh Shah Mohammadi Dermani 3
  • Farshad Ghazalian 2
1 PhD Student of Physical Education and Sports Sciences, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
2 Associate Professor, Department of Physical Education and Sports Sciences, Faculty of Literature, Humanities and Social Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran.
3 Assistant Professor, Department of Obstetrics and Gynecology, Faculty of Medicine, Islamic Azad University of Medical Sciences, Tehran, Iran.
چکیده [English]

Introduction: Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women which is associated with insulin resistance (IR). Some Factors such as adiponectin and APPL-1 play a role in IR signaling that is affected by exercise. Therefore, the present study was conducted with aim to evaluate the effect of swimming training on the IR and the expression of adiponectin and APPL1 genes following induced PCOS in rats.
Methods: This experimental research was conducted in 2021 on 36 female wistar rats. The rats were divided in six groups. Swimming training was done (six and eight weeks/five days a week, 60 min each session/ flow of the water speed was increased from seven to 15 L/M). After 24hr of sacrifices the mice, the level of IR, expression of adiponectin and APPL1 genes were measured in the liver tissue. Data were analyzed using SPSS software (version 22) and two-way ANOVA. P< .05 was considered statistically significant.
Results: Six weeks of training had a significant effect on IR serum levels (P=0.001) and adiponectin gene expression (P=0.004), while it had no significant effect on APPL-1 gene expression (P=0.433). Also, eight weeks of exercises had a significant effect on the IR (P=0.001) and the expression of APPL-1 and adiponectin genes (P=0.001).
Conclusion: Swimming training can lead to significant changes in IR serum levels, expression of adiponectin and APPL-1 genes. It seems that 8 weeks if swimming training have positive effects in PCOS patients by improving the metabolic pathway.

کلیدواژه‌ها [English]

  • Adiponectin
  • APPL-1
  • Insulin Resistance
  • Polycystic Ovary Syndrome
  • Swimming

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مجله زنان، مامایی و نازایی ایران
دوره 26، شماره 1
فروردین 1402
صفحه 40-53

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