بررسی اثر شربت ماءالعسل مرکب بر سندرم تخمدان پلی‌کیستیک القایی توسط استرادیول والرات در موش صحرایی ماده

نوع مقاله : اصیل پژوهشی

نویسندگان

1 مرکز تحقیقات طب سنتی و مفردات پزشکی، دانشکده طب سنتی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران. استادیار گروه طب سنتی، دانشکده طب سنتی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران

2 مرکز تحقیقات طب سنتی و مفردات پزشکی، دانشکده طب سنتی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران. دکترای تخصصی طب سنتی، دانشکده طب سنتی، دانشگاه علوم پزشکی قم، قم، ایران.

3 دانشیار گروه طب سنتی، دانشکده طب سنتی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران.

4 استادیار گروه ایمونولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان، زنجان، ایران.

5 دکترای تخصصی پاتولوژی دامپزشکی، گروه دامپزشکی، دانشگاه آزاد اسلامی واحد شبستر، شبستر، ایران.

6 مرکز تحقیقات طب سنتی و مفردات پزشکی، دانشکده طب سنتی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران.

7 مرکز تحقیقات طب سنتی و مفردات پزشکی، دانشکده طب سنتی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران. استادیار گروه طب سنتی، دانشکده طب سنتی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران.

چکیده

مقدمه: سندرم تخمدان پلی­کیستیک در 10-5% زنان سنین باروری وجود دارد و با اختلال قاعدگی، هیپرآندروژنیسم و کیست تخمدان مشخص می‌گردد. فرآورده­های طب سنتی ایرانی می­تواند در درمان این سندرم، مؤثر باشد. مطالعه حاضر با هدف بررسی اثر شربت ماءالعسل مرکب بر سندرم تخمدان پلی‌کیستیک القایی توسط استرادیول والرات در موش صحرایی ماده انجام شد.
روش‌کار: در این مطالعه تجربی که توسط مرکز تحقیقات طب سنتی و مفردات پزشکی دانشگاه علوم پزشکی شهید بهشتی و در سال 1396 در آزمایشگاه حیوانات دانشگاه علوم پزشکی شهید بهشتی بر روی موش‌های صحرایی ماده انجام شد، از شربت ماءالعسل مرکب (عسل، دارچین، زعفران، خولنجان، زنجبیل، هل، جوزبوا، بسباسه، مصطکی) استفاده شد. 30 سر موش صحرایی ماده به 5 گروه 6­‌تایی کنترل منفی، کنترل مثبت و سه گروه درمانی (دریافت‌کننده ماءالعسل با دوز 1 میلی‌‌لیتر/کیلوگرم، ماءالعسل با دوز 2 میلی‌‌لیتر/کیلوگرم و متفورمین) تقسیم شدند. سندرم تخمدان پلی‌کیستیک با تزریق استرادیول والرات القاء شد. در پایان مداخله موش­ها یوتانازی شده و اندازه­گیری هورمون­ها و بررسی بافت­شناسی تخمدان انجام شد. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS (نسخه 24) و آزمون‌های کروسکال-والیس و آزمون تعقیبی دان-بونفرونی انجام شد. میزان p کمتر از 05/0 معنادار در نظر گرفته شد.
یافته­ ها: در پایان مطالعه، وزن تخمدان در گروه متفورمین و ماءالعسل 2 بالاتر از گروه بدون درمان بود. در وزن بدن و چربی دور شکمی تغییری حاصل نشد. افزایش میزان پروژسترون در هر سه گروه درمانی (002/0=p) و کاهش میزان استروژن در گروه متفورمین نسبت به بدون درمان (021/0=p)، به­طور معناداری مشاهده شد. در بررسی نمونه‌های بافتی تخمدان، کاهش تعداد کیست­های تخمدان در گروه ماءالعسل 1 (001/0=p)، و افزایش تعداد جسم زرد و فولیکول‌های انترال در گروه‌های ماءالعسل 1 و 2 نسبت به گروه بدون درمان معنادار بود (010/0=p). تعداد فولیکول گراف در گروه‌ها تفاوت معناداری نداشت (083/0=p).
نتیجه­ گیری: ماءالعسل مرکب در بهبود وضعیت هورمونی و بافت­شناسی در سندرم تخمدان پلی­کیستیک به اندازه متفورمین و در مواردی بیشتر از آن مؤثر است.
 

کلیدواژه‌ها


عنوان مقاله [English]

Effects of Compound Honey Syrup (Ma-ol-asal) on Polycystic Ovary Syndrome (PCOS) Induced by Estradiol Valerate in Female Rats

نویسندگان [English]

  • Mojgan Tansaz 1
  • Maryam Bahman 2
  • Mahmoud Khodadoost 3
  • Seyyed Shamsadin Athari 4
  • Saadat Ghafarzadeh 5
  • Hanieh Kashafroodi 6
  • Rasool Choopani 7
1 Traditional Medicine & Matria Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Assistant Professor, Department of Traditional Medicine, School of Traditional
2 Traditional Medicine & Matria Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Ph.D of Traditional Medicine, School of Traditional Medicine, Qom University of
3 Associate Professor, Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4 Assistant Professor, Department of Immunology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
5 Ph.D of Veterinary Pathology, Department of Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
6 Traditional Medicine & Matria Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
7 Traditional Medicine & Matria Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Assistant Professor, Department of Traditional Medicine, School of Traditional
چکیده [English]

Introduction: Polycystic ovary syndrome affects 5-10% of women of reproductive age and is characterized by irregular menstruation, hyperandrogenism, and ovarian cysts. Iranian traditional medicine products can be effective in treatment of this syndrome. This study was performed with aim to evaluate the effect of compound honey syrup on the polycystic ovary syndrome induced by estradiol valerate in female rats.
Methods: In this experimental study conducted by traditional medicine and matria medica research center of Shahid Beheshti University of Medical Sciences in animal laboratory of Shahid Beheshti University of Medical Sciences on female rats in 2017, the compound honey syrup (Ma-ol-asal) (containing honey, cinnamon, ginger, saffron, cardamom, galangal, nutmeg, mace and mastic) was used. Thirty female rats were classified into five groups of cases: negative control, positive control, and three treatment groups (receiving two doses of compound honey syrup (1ml/kg and 2ml/kg) and metformin). Polycystic ovary syndrome was induced by injection of Estradiol valerate. At the end of intervention, the rats were euthanized, hormones were measured, and the ovarian histology was examined. Data were analyzed by SPSS software (version 24) and Kruskal-Wallis and Dunn-Bonferroni tests. p < 0.05 was considered statistically significant.
Results: At the end of the study, weights of ovaries in the metformin and Ma-ol-asal 2 groups were higher than the untreated group. There was no change in body weight or abdominal fat. There was a significant increase in levels of progesterone in three treatment groups (P=0.002) and significant reduction of estrogen in the metformin group compared to the untreated group (P=0.021). There was significant reduction of number of ovary cysts in Ma-ol-asal 1 group (P=0.001), and significant increase in the number of Corpus luteum, and antral follicles in Ma-ol-asal 1 and 2 groups compared to the untreated group (P=0.010). The number of graph follicles did not differ significantly in the groups (P=0.083).
Conclusion: Compound honey syrup was effective in improving hormonal and histological status in polycystic ovary syndrome as much as metformin and was more effective in some cases.

کلیدواژه‌ها [English]

  • Compound honey syrup
  • metabolic syndrome
  • Polycystic ovary syndrome
  • Traditional Persian Medicine
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