بررسی همراهی بین پلی‌مورفیسمhas-miR-125 rs12976445 در استعداد ابتلاء به سقط مکرر با دلایل ناشناخته در زنان ایرانی

نوع مقاله : اصیل پژوهشی

نویسندگان

1 دانشجوی دکتری زیست‌شناسی سلولی مولکولی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد سیرجان، سیرجان، ایران.

2 استادیار گروه میکروب‌شناسی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد بافت، بافت، ایران.

3 دانشیار گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه پیام نور، تهران، ایران.

چکیده

مقدمه: بسیاری از مطالعات جدید در ارتباط با دوره پسا-لانه‌گزینی و بقای بارداری، حاکی از آن است که microRNA‌ها نقش اساسی در تنظیم مسیرهای زودرس منجر به سقط و نارسایی در لانه‌گزینی را بر عهده دارند. miR-125 بر روی کروموزوم 19q13.41 قرار گرفته و نقش مهمی در تکامل ارگان دارد. دو ژن هدف miR-125، شامل ERBB2 و LIF در تمایز سلول‌های تروفوبلاستی و رگ‌زایی درگیر می‌باشند. مطالعه حاضر با هدف بررسی ارتباط احتمالی پلی‌مورفیسم has-miR-125 T> C در استعداد ابتلاء به سقط مکرر با دلایل ناشناخته در زنان ایرانی انجام شد.
روش‌کار: در این مطالعه مورد- شاهدی 116 زن با سابقه حداقل دو سقط مکرر و 89 زن غیر‌مبتلا با سابقه داشتن دو بارداری موفق و بدون سابقه سقط مورد مطالعه قرار گرفتند. مولکول DNA با استفاده از روش RGDE تخلیص و سپس ژنوتایپینگ با استفاده از روش Tetra ARMS اجرا گردید. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار SPSS (نسخه 22) و آزمون کای دو انجام شد. میزان p کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته‌ها: در مطالعه حاضر فراوانی ژنوتیپ‌هایTT ، TC و CC در پلی‌مورفیسم rs12976445 در زنان مبتلا به سقط به‌ترتیب 86/50%، 25/42% و 89/6% و در زنان سالم 43/58%، 33/39% و 24/2% بود. بین پلی‌مورفیسم rs12976445 و سقط مکرر هیچ ارتباط معنی‌داری مشاهده نشد (238/0=p).
نتیجه‌گیری: بین پلی‌مورفیسم rs12976445 و سقط مکرر خودبه‌خودی ارتباط معنی‌داری وجود ندارد.

کلیدواژه‌ها

عنوان مقاله [English]

Association between polymorphism of has-miR-125 (rs12976445) and susceptibility to idiopathic recurrent pregnancy loss in Iranian women

نویسندگان [English]

  • Sara Nemati Vahedi 1
  • Babak Kheirkhah 2
  • Ali Akbar Malekirad 3
1 PhD student in Molecular Cell Biology, Faculty of Basic Sciences, Islamic Azad University Sirjan Branch, Sirjan, Iran.
2 Assistant Professor, Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University Baft Branch, Baft, Iran.
3 Associate Professor, Department of Biology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran.
چکیده [English]

Introduction: Many new studies related to peri-implantation period and maintenance of pregnancy suggest that microRNAs have fundamentally role in regulating critical pathways in early pregnancy loss and implantation failure. This study was performed with aim to evaluate the possible association between has-miR-125 T>C polymorphism and susceptibility to idiopathic recurrent pregnancy loss (RPL).
Methods: In this case-control study, 116 females with at least two or more recurrent pregnancy loss and 89 healthy women with a history of two successful pregnancies and without a history of miscarriage were evaluated. DNA molecule was extracted by RGDE method and genotyping was performed by ARMS Tetra method. Data were analyzed by SPSS software (version 22) and Chi-squared test. P<0.05 was considered statistically significant.
Results: The frequency of TT, TC and CC genotypes of rs12976445 polymorphism were 50.86%, 42.25% and 6.89% and in women with RPL and were 58.43%, 39.33% and 2.24% in healthy women. There was no significant relationship between rs12976445 polymorphism and recurrent pregnancy loss (P=0.238).
Conclusion: No significant association was found between rs12976445 polymorphism and RPL.

کلیدواژه‌ها [English]

  • miR-125
  • Recurrent pregnancy loss (RPL)
  • Single nucleotide polymorphism (SNP)
  • Tetra -ARMS PCR

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مجله زنان، مامایی و نازایی ایران
دوره 23، شماره 8
آبان 1399
صفحه 37-48

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