بررسی همراهی پلیمورفیسم rs4648551 ژن P73 در زنان ایرانی مبتلا به سقط مکرر با دلایل ناشناخته

نوع مقاله : اصیل پژوهشی

نویسندگان

1 کارشناس ارشد ژنتیک، دانشکده علوم زیستی، دانشگاه آزاد اسلامی واحد تهران شمال، تهران، ایران.

2 استادیار گروه ژنتیک پزشکی، مرکز تحقیقات ژنتیک انسانی، پژوهشکده فناوری ژن و سلول، دانشگاه علوم پزشکی بقیه‌الله (عج)، تهران، ایران.

چکیده

مقدمه: سقط‌های مکرر خودبه‌خودی به‌عنوان یک رویداد تکرارپذیر از دو یا بیشتر سقط قبل از هفته 20 تعریف می‌شود. در سقط مکرر به‌عنوان یک بیماری چندعاملی، مسائل متعددی دخیل است و نقص ژنتیکی به‌عنوان یکی از عوامل عمده خطر برای سقط مکرر مطرح می‌باشد. با توجه به نقش مؤثر ژن p73 در فرآیند تقسیم بلاستوسیست اولیه و حفاظت از سلول‌های جنسی، به‌نظر می‌رسد پلی‌مورفیسم rs4648551 در این ژن p73، با بروز علائم سقط مکرر خود‌به‌خودی ارتباط دارد. مطالعه حاضر با هدف مقایسه فراوانی این پلی‌مورفیسم بین دو گروه زنان ایرانی مبتلا به سقط مکرر با دلایل ناشناخته و زنان سالم اجرا شد.
روش‌کار: این مطالعه تحلیلی از نوع مورد - شاهدی در سال 95-1394 بر روی 90 بیمار که همگی دارای سابقه 2 سقط مکرر خود‌به‌خودی متوالی بدون علت مشخص در مقایسه با 110 نفر از افراد سالم که دارای حداقل 2 بارداری موفق بودند، به‌عنوان کنترل انجام شد. DNA با روش RGDE استخراج و ژنوتایپنگ با روش Tetra ARMS اجرا گردید. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS (نسخه 22) و آزمون کای دو انجام شد. میزان p کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته‌ها: در پلی‌مورفیسم (rs4648551 A<G)، فراوانی ژنوتیپ‌های GG، AG و AA در ژن p73 در زنان گروه کنترل 81/11%، 09/69% و 09/19% و در زنان گروه سقط 11/31%، 67/46% و 22/22% بود که تفاوت‌ها از لحاظ آماری معنا‌دار بود (05/0>p)، اما مقدار تفاوت آللی بین گروه بیمار و کنترل 066/0 به‌دست آمد که معنی‌دار نبود و مطابق نتایج به‌دست آمده، مدل توارث غالبی پیشنهاد شد (05/0>p).
نتیجه‌گیری: بین پلی‌مورفیسم rs4648551 و سقط مکرر ایدیوپاتیک ارتباط معنی‌داری وجود دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Association of rs4648551 polymorphism in p73 gene in Iranian woman with idiopathic recurrent pregnancy loss

نویسندگان [English]

  • Somayeh Tabasi 1
  • Seyed Mostafa Hoseini 2
1 M.Sc. of Genetics, School of Biology Sciences, Tehran-north Branch, Islamic Azad University, Tehran, Iran.
2 Assistant professor, Department of Medical Genetics, Human Genetic Research Center, Gene & Cell Technology Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
چکیده [English]

Introduction: Recurrent pregnancy loss (RPL) is defined as the repeated occurrence of 2 or more miscarriages before 20th week of gestation. RPL is a multifactorial disorder and genetic defects are considered as one of the major risk factors for recurrent miscarriage. Regarding to the effective role of p73 gene in the process of primary blastocyst division and protection of germ cells, it seems that rs4648551 polymorphism in this p73 gene be associated with the occurrence of spontaneous abortion symptoms. This study was performed with aim to evaluate the frequency of this polymorphism between two groups of Iranian women with idiopathic recurrent miscarriage and healthy women.
Methods: This descriptive case-control study was performed in 2015-2016 on 90 patients with history of 2 consecutive idiopathic recurrent spontaneous miscarriages compared to 110 healthy women who had at least two successful pregnancies as controls. DNA was extracted by RGDE procedure and genotyping was performed by Tetra ARMS technique. Data were analyzed by SPSS software (version 22) and Chi-square test. p < 0.05 was considered statistically significant.
Results: In SNP rs4648551(A<G), the frequency of GG, CC and GC alleles in p73 gene in the control group were 11.81%, 69.09% and 19.09%, respectively and in the RPL group were 31.11%, 46.67% and 22.22%, respectively; the difference was statistically significant (p < 0.05). But, the value of allelic difference between the patient and control groups was calculated as 0.066 which was not significant. According to the results, the dominant heredity model was suggested (p < 0.05).
Conclusion: There is significant association between polymorphism rs4648551 and idiopathic recurrent abortions.

کلیدواژه‌ها [English]

  • Polymorphism
  • P73 gene
  • Recurrent Pregnancy Loss (RPL)
  • Single nucleotide polymorphism (SNP)
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