اثرات درمانی عصاره آبی- الکلی برگ اسفناج آبی در مقابل سمیت ناشی از مصرف بوسولفان بر پارامترهای باروری در موش صحرایی نر؛ یک مطالعه کارآزمایی بالینی تصادفی شاهددار

نوع مقاله : اصیل پژوهشی

نویسندگان

1 دانشیار گروه علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه، ایران.

2 دانشیار گروه پرستاری، پژوهشکده سلامت، دانشکده پرستاری، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه، ایران.

3 دانشجوی دکترای علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی کرمانشاه، کرمانشاه، ایران.

چکیده

مقدمه: داروی بوسولفان جهت معالجه سرطان، به‌علت خواص اکسیداسیونی و تولید رادیکال­های آزاد سبب اختلال در پارامترهای باروری مردانه می­شود و برگ گیاه اسفناج آبی دارای خواص آنتی‌اکسیدانی است. مطالعه حاضر با هدف ارزیابی اثر برگ اسفناج آبی بر بهبود آسیب‌های دستگاه تناسلی نر ناشی از بوسولفان انجام شد.
روش­کار: این مطالعه کارآزمایی بالینی تصادفی شاهددار در سال 1398 بر روی 48 موش صحرایی نر نژاد ویستار انجام شد. نمونه‌ها در 8 گروه شامل: نرمال سالین، بوسولفان، عصاره برگ اسفناج آبی با دوزهای 250، 500 و 1000 میلی‌گرم/کیلوگرم و عصاره + بوسولفان قرار گرفتند. تیمار روزانه و طی 2 ماه به‌صورت داخل صفاقی انجام شد. پارامترهای اسپرم با استفاده از تکنیک­های رنگ‌سنجی و توسط میکروسکوپ نوری، سطح مالون دی‌آلدهید بیضه توسط اسپکتروفتومتری، ظرفیت آنتی‌اکسیدان تام و تستوسترون با روش الایزا و ارتفاع لایه سمینی فروس از طریق میکروسکوپ نوری و نرم‌افزار موتیک اندازه‌گیری شد. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS (نسخه 20) و آزمون‌ تکمیلی توکی انجام شد. میزان p کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته­ها: مقادیر پارامترهای اسپرم، هورمون تستوسترون و ظرفیت آنتی‌اکسیدان تام در گروه کنترل منفی نسبت به کنترل مثبت به‌طور معنی‌داری کاهش یافت (01/0>p). تیمار توسط عصاره و عصاره+ بوسولفان در تمامی دوزها، به‌طور معنی­داری موجب افزایش تمامی پارامترهای ذکر شده نسبت به گروه کنترل منفی شد (01/0>p). در پارامترهای بررسی شده در گروه­ عصاره به‌تنهایی، تغییر معنی‌داری نسبت به گروه کنترل مثبت مشاهده نشد (05/0<p).
نتیجه­گیری: عصاره برگ اسفناج کیفیت اسپرم را افزایش داده و اثرات سوء بوسولفان را بر روی پارامترهای تولید مثلی نر بهبود می­بخشد.

کلیدواژه‌ها

عنوان مقاله [English]

Therapeutic effects of hydro-alcoholic extract of water spinach leaf against toxicity following Busulfan administration on fertility parameters in male rats; A randomized controlled clinical trial study

نویسندگان [English]

  • Mohammad Reza Salahshoor 1
  • Shiva Roshankhah 1
  • Amir Jalali 2
  • Amir Abdolmaleki 3
1 Associate professor, Department of Anatomical Sciences, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
2 Associate professor, Department of Nursing, Research Institute for Health, School of Nursing, Kermanshah University of Medical Sciences, Kermanshah, Iran.
3 PhD candidate of Anatomical Sciences, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
چکیده [English]

Introduction: Busulfan drug for cancer treatment, due to the oxidation properties and free radicals production, causes disturbance on male fertility parameters, and leaves water spinach plant have antioxidant features. The current study was aimed to evaluate the effect of water spinach leaf on recovery of male genital injuries caused by Busulfan.
Methods: This randomized controlled clinical trial was performed on 48 male rats of Wistar race in 2019. The specimens were divided into 8 groups including; normal saline, Busulfan, water spinach leaf extract with doses of 250, 500, and 1000 mg/kg, extract + Busulfan. Daily treatment for 2 months was administered intraperitoneally. Sperm parameters by colorimetric techniques and light microscopy, level of testis malondialdehyde by spectrophotometry, total antioxidant capacity and testosterone by ELISA method, and height of germinal layer through optical microscopy and Motic software were evaluated. Data analysis was applied by SPSS software (version 20) and Tukey post-hoc test. P< 0.05 were considered statistically significant.
Results:The values of sperm parameters, testosterone hormone, and total antioxidant capacity were significantly decreased in negative control group than positive control (P < 0.01). Treatment by extract and extract + Busulfan in all doses significantly increased whole mentioned parameters compared to the negative control group (P < 0.01). In evaluated parameters in extract group alone, no significant changes were observed compared to the positive control group (P > 0.05).
Conclusion: Leaf extract of water spinach plant increases the sperm quality and improves the Busulfan-induced adverse effects on male reproductive parameters.

کلیدواژه‌ها [English]

  • Water spinach
  • Fertility
  • Busulfan
  • Clinical trial

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مجله زنان، مامایی و نازایی ایران
دوره 23، شماره 6
شهریور 1399
صفحه 17-28

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