تفاوت در شیوع ساب‌تایپ‌های پاپیلوما ویروس انسانی نسبت به دانسته‌های قبلی بر اساس تکنیک واکنش زنجیره‌ای پلیمراز مولکولی در زنان دارای پاپ‌اسمیر سالم

نوع مقاله : اصیل پژوهشی

نویسندگان

1 پزشک عمومی، دانشکده پزشکی، دانشگاه علوم پزشکی آزاد اسلامی واحد تهران، تهران، ایران.

2 استادیار گروه زنان و مامایی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید بهشتی، تهران، ایران.

10.22038/ijogi.2025.85084.6306

چکیده

مقدمه: با توجه به شیوع و عوارض بالای ویروس پاپیلومای انسانی (HPV) و اهمیت بالای تشخیص ابتلاء و نوع ژنوتیپ مسبب و نیز عوامل مؤثر بر آن، مطالعه حاضر با هدف تعیین شیوع HPV و ارتباط آن با خصوصیات جمعیت­شناختی زنان دارای پاپ اسمیر سالم انجام شد.
روش‌کار: در این مطالعه­ تحلیلی- مقطعی، 225 زن با پاپ‌اسمیر سالم مراجعه­ کننده به بیمارستان ­های تابعه دانشگاه علوم پزشکی آزاد اسلامی تهران در سال­ های 1403-1401، با توجه به معیارهای ورود وارد مطالعه شدند. ابتلاء به HPV و ژنوتیپ آن با روش Real-Time PCR تعیین و ارتباط آن­ها با ویژگی­ های جمعیت ­شناختی بررسی شد. تجزیه و تحلیل داده ­ها با استفاده از نرم ­افزار آماری SPSS (نسخه 23) و آزمون‌های آماری تی استیودنت، من­ ویتنی و کای دو انجام شد. میزان p کم­تر از 05/0 معنی ­دار در نظر گرفته­ شد.
یافته ­ها: در مجموع 63 نفر (0/28%) مبتلا به HPV بودند که بیش­تر افراد یعنی 35 نفر (5/55%) دچار انواع با خطر بالا بودند. در گروه انواع با خطر پایین، ساب­ تایپ 6 (0/25%)، در انواع با خطر متوسط، ساب­ تایپ 53 (8/24%) و 73 (8/24%) و در انواع با خطر بالا، ساب­ تایپ 51 (0/20%)، شایع‌ترین بود. فقط بین سطح تحصیلات با انواع مختلف HPV ارتباط معنی­ داری وجود داشت (037/0=p).
نتیجه ­گیری: شیوع انواع HPV و خصوصاً ساب ­تایپ ­های ناشناخته ­تر (51، 53 و 73) در زنان دارای پاپ اسمیر سالم، قابل توجه بوده که بررسی آن در جوامع مختلف با روش PCR، خصوصاً در افراد با سطح تحصیلات پایین­ تر ضروری است.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Difference in the Prevalence of Human Papillomavirus Subtypes Compared to Previous Knowledge based on Molecular Polymerase Chain Reaction Technique in Women with Normal Pap Smears

نویسندگان [English]

  • Shayan Sadrinasab 1
  • Mahmoud Pourghadiri Isfahani 1
  • Sadaf Saket 2
  • Saba Saket 1
1 General Practitioner, Faculty of Medicine, Islamic Azad University of Medical Sciences, Tehran Branch, Tehran, Iran.
2 Assistant Professor, Department of Obstetrics and Gynecology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
چکیده [English]

Introduction: Given the high prevalence and complications of human papillomavirus (HPV) and the high importance of diagnosing the infection and the type of causative genotype, as well as the factors affecting it, this study was conducted with aim to determine the prevalence of HPV and its relationship with demographic characteristics of women with normal Pap smears.
Methods: In this cross-sectional analytical study, 225 women with normal Pap smears referred to the hospitals affiliated with Tehran Islamic Azad University of Medical Sciences from 2022 to 2024 were included in the study according to the inclusion criteria. HPV infection and its genotype were determined by Real-Time PCR method and their relationship with demographic characteristics was examined. Data were analyzed by SPSS software (version 23) and student t, Mann-Whitney, and Chi-square tests. P<0.05 was considered significant.
Results: In total, 63 subjects (28.0%) were infected with HPV, most of whom (n=35, 55.5%) had high-risk types. In the low-risk group, subtype 6 (25%), in the intermediate-risk group, subtypes 53 (24.8%) and 73 (24.8%), and in the high-risk group, subtype 51 (20%) were the most common. There was only a significant association between education level and different HPV types (p=0.037).
Conclusion: The prevalence of HPV types, especially the less known subtypes (51, 53, and 73), in women with healthy Pap smears was significant, and it is necessary to investigate it in different communities, especially in people with lower education level, by PCR method.

کلیدواژه‌ها [English]

  • Human papillomavirus
  • Molecular polymerase chain reaction
  • Pap smear

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مجله زنان، مامایی و نازایی ایران
دوره 28، شماره 4
تیر 1404
صفحه 29-36

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