پاسخ بیان ژن های NR5A1 و CYP19A1 بافت تخمدان رت های مدل تجربی اندومتریوز به فعالیت ورزشی منظم و مکمل آنتی اکسیدانی

نوع مقاله : اصیل پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

2 استاد، گروه فیزیولوژی ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

3 استادیار، گروه فیزیولوژی ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

4 استادیار، گروه آسیب‌شناسی ورزشی و حرکات اصلاحی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

10.22038/ijogi.2025.77772.5984

چکیده

مقدمه: شنا می‌تواند با کاهش سطح التهاب و استرس اکسیداتیو به بهبود علائم بیماری اندومتریوز کمک کند. همچنین، ویتامین­ های C و E ممکن است در پاک‌سازی رادیکال ­های آزاد در زنان مبتلا به اندومتریوز دخیل باشند. علاوه بر این، ویتامین‌های B، هم متابولیسم استروژن را به شکل غیرفعال افزایش می­ دهند و هم از تبدیل اسید لینولئیک به گامالینولنیک اسید پشتیبانی می‌کنند. مطالعه حاضر با هدف بررسی اثر تمرین منظم هوازی و ویتامین­ های آنتی‌اکسیدانی بر بیان ژن ­های NR5A1 و CYP19A1 بافت تخمدان رت­ های مدل اندومتریوز انجام شد.
روش‌کار: در این تحقیق تجربی 50 سر موش صحرایی ماده به 10 گروه: 1- کنترل سالم، 2- اندومتریوز، 3- شم، 4- اندومتریوز + تمرین، 5- اندومتریوز + ویتامین B، 6- اندومتریوز + امگا-3، 7- اندومتریوز + ویتامین E، 8- تمرین+ اندومتریوز+ ویتامین B، 9- تمرین+ اندومتریوز+ ویتامین E و 10- تمرین+ اندومتریوز+ امگا-3 تقسیم شدند. برنامه تمرین شامل 5 روز شنا کردن در هفته و به‌مدت 8 هفته بود. ویتامین E، مکمل امگا-3 و ویتامین B (ترکیبی از ویتامین‌های B6، B2، B1) به شکل گاواژ دریافت شد. بیان ژن CYP19A1و NR5A1 با روش real-time PCR اندازه‌گیری گردید. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS (نسخه 23) و آزمون آنووا در سطح معنی‌داری 05/0 p<انجام شد.
یافته ­ها: القای مدل تجربی اندومتریوز منجر به افزایش معنی­ دار ژن NR5A1 نسبت به گروه کنترل سالم شد (001/0>p). تمرین شنا+ ویتامین E (013/0=p) و تمرین شنا+ امگا-3 (012/0=p) منجر به کاهش معنی­ دار ژن NR5A1 بافت تخمدان در رت­ های مدل تجربی اندومتریوز شد. القای مدل تجربی اندومتریوز نیز منجر به افزایش معنی ­دار ژن CYP19A1 نسبت به گروه کنترل سالم شد (003/0=p). گروه ترکیبی تمرین+ امگا-3 منجر به کاهش معنی‌دار ژن CYP19A1 بافت تخمدان نسبت به گروه اندومتریوز شد (031/0=p).
نتیجه­ گیری: اندومتریوز با افزایش بیان ژن­ های NR5A1 و CYP19A1 بافت تخمدان همراه است و انجام تمرین شنا به همراه مصرف امگا-3 و ویتامین E با کاهش بیان ژن­ های NR5A1 و CYP19A1، به بهبود اندومتریوز کمک خواهد کرد. 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Response of NR5A1 and CYP19A1 genes of ovarian tissue in endometriosis experimental model rats to regular exercise and antioxidant supplement

نویسندگان [English]

  • Sakineh Sadeghian 1
  • Parvin Farzanegi 2
  • Amin Farzaneh Hesari 3
  • Yasaman Asadi Saravi 4
1 PhD Student in Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
2 Professor, Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
3 Assistant Professor, Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
4 Assistant Professor, Department of Sport Rehabilitation and Corrective Exercise, Sari Branch, Islamic Azad University, Sari, Iran.
چکیده [English]

Introduction: Swimming can help improve symptoms of endometriosis by reducing inflammation and oxidative stress. Also, vitamins C and E may be involved in scavenging free radicals in women with endometriosis. In addition, vitamins B both increase estrogen metabolism in a passive form and support the conversion of linoleic acid to gamma-linolenic acid. The present study was conducted with aim to investigate the effect of regular aerobic exercise and antioxidant vitamins on the expression of NR5A1 and CYP19A1 genes in ovarian tissue of endometriosis model rats.
Methods: In this experimental study, 50 female rats were divided into 10 groups: 1-healthy control, 2-endometriosis, 3-sham, 4-endometriosis+exercise, 5-endometriosis+vitamin B, 6-endometriosis+omega3, 7-endometriosis+vitaminE, 8-exercise+endometriosis+vitaminB, 9-exercise+endometriosis+vitaminE, 10-exercise+endometriosis+omega3. The training program consisted of swimming 5 days a week for 8 weeks. Vitamin E was administered at a dose of 200mg/kg, omega3 supplement at a dose of 2ml/kg, and vitamin B (a combination of vitamins B6, B2, B1) at a dose of 60 mg/kg per day by gavage. CYP19A1 and NR5A1 gene expression was measured by real-time PCR. Data were analyzed using SPSS software (version 23) and ANOVA test. P<0.05 was considered significant.
Results: The induction of the experimental model of endometriosis caused a significant increase in the NR5A1 gene compared to the healthy control group (p<0.001). Swimming training+vitamin E (p=0.013) and swimming training+omega3 (p=0.012) led to a significant decrease of NR5A1 gene in ovarian tissue in the experimental model of endometriosis rats. The induction of the experimental model of endometriosis also caused a significant increase in the CYP19A1 gene compared to the healthy control group (p=0.003). The combined exercise+omega3 group had a significant decrease in ovarian CYP19A1 gene compared to the endometriosis group (p=0.031).
Conclusion: Endometriosis is associated with increased NR5A1 and CYP19A1 genes in the ovarian tissue and swimming training plus omega3 and vitamin E will help to improve endometriosis by reducing NR5A1 and CYP19A1 gene expression.

کلیدواژه‌ها [English]

  • Antioxidant
  • Endometriosis
  • Exercise

مراجع

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مجله زنان، مامایی و نازایی ایران
دوره 27، شماره 11
بهمن 1403
صفحه 26-37

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