شناسایی شبکه تنظیمی عمکردی miRNA-mRNA در سقط مکرر: آنالیز بیوانفورماتیکی پرزهای جفتی انسانی

نوع مقاله : اصیل پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد، گروه ژنتیک، دانشکده علوم پایه، دانشگاه شهرکرد، شهرکرد، ایران.

2 دانشیار، گروه ژنتیک، دانشکده علوم پایه، دانشگاه شهرکرد، شهرکرد، ایران.

10.22038/ijogi.2025.82344.6191

چکیده

مقدمه: سقط خود‌به‌خودی مکرر (RSA)، یک اختلال تولیدمثلی است که مسئله حل نشده‌ای را در زنان ایجاد می‌کند. miRNAها ممکن است در تنظیم رشد جنین و حفظ بارداری نقش داشته باشند. مطالعه حاضر با هدف بررسی پروفایل miRNA در پرز جفتی زنان با سقط مکرر با مطالعات بیوانفورماتیکی انجام شد.
روش‌کار: در این مطالعه، پروفایل بیانی mRNA و miRNA سلول‌های پرز جفتی از پایگاه GEO دریافت شدند. بعد از به‌دست آوردن هدف‌های miRNAهای دارای تغییر بیان، اشتراک این هدف ­ها با mRNAهای کاهشی/افزایشی به‌دست آمد. در مرحله بعد هویت‌شناسی ژنی (GO) و مسیرهای KEGG، برای ژن ­های مشترک انجام شد. شبکه میانکنش پروتئین- پروتئین و ژن­ های کلیدی مورد بررسی قرار گرفت. در نهایت شبکه تنظیمی miRNA-mRNA رسم شد.
یافته ­ها: تعداد 123 miRNA با تغییر بیان (16 عدد کاهش بیان و 107 عدد افزایش بیان) و 670 ژن با تغییر بیان (شامل 269 ژن کاهش بیان و 366 ژن با افزایش بیان) آنالیز شدند. بعد از ایجاد اشتراک میان هدف‌های miRNAها و ژن‌های با تغییر بیان، 219 ژن مشترک به‌دست آمد. نتایج آنالیز KEGG و GO نشان داد که ژن‌ها در مسیرهای پرولاکتین، اکسی‌توسین، تولید اینترلوکین 6 و تمایز لوکوسیتی نقش دارند. همچنین در شبکه miRNA-mRNA ترسیم شده، miR-548ap-5p و miR-320b با بیشترین امتیاز مشخص شدند.
نتیجه ­گیری: مطالعه حاضر miRNA­ها و mRNAهای دارای تغییر بیان و  همچنین ژن‌های کلیدی در سلول­ های پرز جفتی با RSA را شناسایی کرد. این مطالعه می‌تواند مارکرهای زیستی جدیدی را برای تشخیص یا درمان احتمالی RSA پیشنهاد کند.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Identification of a functional miRNA–mRNA regulatory network in recurrent miscarriage: bioinformatics analysis of human chorionic villi

نویسندگان [English]

  • Yass Rohani 1
  • Somayeh Raeisi 2
1 M.Sc. Student, Department of Genetics, School of Basic Sciences, Shahrekord University, Shahrekord, Iran.
2 Associate Professor, Department of Genetics, School of Basic Sciences, Shahrekord University, Shahrekord, Iran.
چکیده [English]

Introduction: Recurrent spontaneous abortion (RSA) is a reproductive disorder that makes an unresolved issue in women. miRNAs may play a role in regulating embryonic growth and sustaining pregnancy. The present study was conducted with aim to investigate the miRNA profile in the chorionic villi of women with recurrent abortion using bioinformatics studies.
Methods: In this study, the expression profiles of mRNA and miRNA from chorionic villi cells were obtained from the GEO database. After identifying the differentially expressed miRNAs, the overlap between miRNA targets and the upregulated/downregulated mRNAs was determined. Gene Ontology (GO) analysis and KEGG pathways were then conducted for the overlapped genes. Protein-protein interaction networks and key genes were analyzed. Finally, the miRNA-mRNA regulatory network was constructed.
Results: A total of 123 miRNAs with differential expression (16 downregulated and 107 upregulated) and 670 genes with differential expression (including 269 downregulated and 366 upregulated) were analyzed. After making the overlap between the miRNA targets and differentially expressed genes, 219 overlap genes were identified. The results of KEGG and GO analysis indicated that these genes are involved in prolactin, oxytocin, B cell receptor pathways, interleukin-6 production processes, and leukocyte differentiation. Additionally, in the miRNA-mRNA network, miR-548ap-5p and miR-320b were identified with the highest scores.
Conclusion: The present study identified differentially expressed miRNAs and mRNAs, as well as key genes in the chorionic villi cells associated with RSA. This study may suggest new biomarkers for the potential diagnosis or treatment of RSA.

کلیدواژه‌ها [English]

  • miRNA-mRNA network
  • Recurrent spontaneous abortion (RSA)
  • Signaling pathway

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مجله زنان، مامایی و نازایی ایران
دوره 27، شماره 10
دی 1403
صفحه 63-76

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