اثر 8 هفته تمرین شنا، سلول‌درمانی و مصرف ویتامین E بر سطوح تستوسترون و ژن‌های درگیر در مسیر اتوفاژی موش‌های نر نابارور

نوع مقاله : اصیل پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزش، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

2 دانشیار، گروه فیزیولوژی ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

3 استادیار، گروه فیزیولوژی ورزشی، واحد ساری، دانشگاه آزاد اسلامی، ساری، ایران.

4 استادیار، گروه فیزیولوژی ورزشی، واحد قائم‌شهر، دانشگاه آزاد اسلامی، قائم‌‌شهر، ایران.

چکیده

مقدمه: ناباروری در واقع عدم باروری در زوجینی است که با وجود یک سال یا بیشتر تلاش برای تولید مثل، هیچ ­گونه لقاحی در آن­ها رخ نداده است. مطالعه حاضر با هدف بررسی اثر 8 هفته تمرین شنا، سلول­درمانی و مصرف ویتامین E بر تستوسترون و ژن­ های Lc31 و P62 بافت بیضه موش ­های مدل آزواسپرمی انجام شد.
روش‌کار: در این مطالعه تجربی، 40 سر رت 6 تا 8 هفته ­ای به ­صورت تصادفی انتخاب، و سپس مدل آزواسپرمی با داروی بوسولفان با دوز 40 میلی­ گرم القاء شد. پس از گذشت یک­ ماه موش ­ها در 8 گروه: 1) کنترل، 2) بیمار، 3) شم، 4) بیمار + تمرین، 5) بیمار + مکمل، 6) بیمار + سلول، 7) بیمار + مکمل + تمرین و 8) بیمار + سلول + تمرین، تقسیم شدند. سلول‌های بنیادی در ناحیه مجران دفران به میزان یک میلیون سلول برای هر موش پیوند زده شد، محلول خوراکی ویتامین E به میزان 100 میلی‌گرم بر کیلوگرم را به ­صورت گاواژ دریافت کردند و هم­چنین گروه ­های تمرینی به­مدت 8 هفته، 30 دقیقه در روز، 5 روز در هفته تمرین شنا را به‌صورت تداومی و با شدت ثابت 60 درصد ضربان قلب بیشینه انجام دادند. سطوح تستوسترون به روش الایزا و بررسی بیان ژن­ های P62 و LC31 بافت بیضه با تکنیک PCR Real Time انجام شد. تجزیه و تحلیل داده­ ها با استفاده از نرم‌افزار آماری SPSS  (نسخه 26) انجام شد. میزان p کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته ­ها: القای آزواسپرمی باعث کاهش معنی ­دار سطوح تستوسترون و بیان ژن ­های Lc31 و P62 بافت بیضه نسبت به گروه کنترل شد (05/0≥p)، اما در گروه  ­های مکمل، سلول، تمرین، تمرین+ مکمل و تمرین+ سلول نسبت به گروه­ های بیمار و شم افزایش معنی­ دار را نشان دادند (05/0≥p).
نتیجه ­گیری: در مطالعه حاضر هم­افزایی تمرین شنا با ویتامین E و سلول بنیادی مزانشیمی مشتق از مغز استخوان در بهبود شار اتوفاژی در رت­ های مدل تجربی آزواسپرمی مشاهده شد که ممکن است از این طریق در افزایش تستوسترون و یا حتی باروری مؤثر باشد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of 8 weeks of swimming training, cell therapy and vitamin E consumption on testosterone levels and genes involved in the autophagy pathway of infertile male rats

نویسندگان [English]

  • Behnam Esmaeeli 1
  • Parvin Farzanegi 2
  • Seyed Abdollah Hashemvarzi 3
  • Babisan Askari 4
1 PhD Student in Sports Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
2 Associate Professor, Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
3 Assistant Professor, Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
4 Assistant Professor, Department of Exercise Physiology, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, Iran.
چکیده [English]

Introduction: Infertility is actually a lack of fertility in couples that has not conceived despite a year or more of trying to reproduce. The present study was performed with aim to investigate the effect of 8 weeks of swimming training, cell therapy and vitamin E consumption on testosterone and Lc31 and P62 genes in the testicular tissue of azoospermia model rats.
Methods: In this experimental study, forty 6- to 8-week-old rats were randomly selected, and then the azoospermia model was induced with the busulfan at a dose of 40 mg. After one month, the rats were divided into 8 groups: 1) control, 2) patient, 3) sham, 4) patient + exercise, 5) patient + supplement, 6) patient + cell, 7) patient + supplement + exercise, and 8) patient + cell + exercise. Stem cells were transplanted in the vas deferens at the rate of one million cells for each mouse, they received an oral solution of vitamin E at the rate of 100 mg/kg by gavage, also the training groups performed swimming training continuously with a constant intensity of 60% of the maximum heart rate for 8 weeks, 30 minutes a day, 5 day of the week. Testosterone levels were measured by ELISA and the expression of P62 and LC31 genes in testicular tissue was measured by Real Time PCR technique. Data were analyzed using SPSS statistical software (version 26). P<0.05 was considered statistically significant.
Results: Induction of azoospermia significantly decreased testosterone levels and the expression of Lc31 and P62 genes in testicular tissue compared to the control group (P≤0.05), but showed a significant increase in the supplement, cell, exercise, exercise+supplement and exercise+cell groups compared to the patient and sham groups (P≤0.05).
Conclusion: In the present study, the synergism of swimming exercise with vitamin E and mesenchymal stem cells derived from bone marrow in improving the autophagy flux in experimental rats with azoospermia was observed, which may be effective in increasing testosterone or even fertility.

کلیدواژه‌ها [English]

  • Azoospermia
  • Autophagy
  • Mesenchymal Stem Cell
  • Swimming Exercise
  • Vitamin E

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مجله زنان، مامایی و نازایی ایران
دوره 27، شماره 3
خرداد 1403
صفحه 37-49

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