تشخیص و جداسازی مایکوپلاسما ژنیتالیوم از ادرار احتباسی زنان باردار به روش PCR

نوع مقاله : اصیل پژوهشی

نویسندگان

1 دانشیار گروه علوم آزمایشگاهی، دانشکده پیراپزشکی، دانشگاه علوم پزشکی تهران، تهران، ایران.

2 کارشناس ارشد میکروب‌شناسی، گروه اپیدمیولوژی، انیستیتو پاستور، تهران، ایران.

چکیده

مقدمه: مایکوپلاسما، یکی از مهم‌ترین عوامل باکتریایی عفونت‌های دستگاه تناسلی در زنان مردان و به‌دنبال آن در مواردی زایمان زودرس و حتی سقط جنین در زنان باردار می‌باشد. مطالعه حاضر با هدف بررسی موارد جداسازی مایکوپلاسما ژنیتالیوم در تعدادی از زنان باردار که در 3 ماهه اول بارداری بودند، انجام شد.
روشکار: در این مطالعه مقطعی (توصیفی- تحلیلی) که در سال‌های 1400-1399 انجام شد، از 66 زن بارداری که برای انجام تست‌های غربالگری سه ماهه اول (بین 14-11 هفتگی) به مرکز بهداشتی درمانی مراجعه کرده بودند، نمونه‌گیری ادرار احتباسی به‌عمل آمد. به این ترتیب که این زنان حداقل از 2 ساعت قبل تخلیه مثانه انجام نداده و نمونه ادرار در ظروف استریل جمع‌آوری شد. DNA رسوب ادرار استخراج شده و از نظر وجود ژنوم مایکوپلاسما تحت آزمایش PCR قرار گرفتند. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS (نسخه 20) و آزمون‌ آماری تی مستقل انجام شد. میزان p کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته ­ها: در مطالعه حاضر میزان جداسازی مایکوپلاسما از نمونه ادرار احتباسی زنان با روش PCR حدود 65/17% بود.
نتیجه ­گیری: به‌دلیل خطراتی که نمونه‌گیری ترشحات واژن و سرویکس برای تشخیص مایکوپلاسما در زنان باردار دارد، نمونه ادرار احتباسی روش ایمن‌تر و مورد اعتمادتری برای تشخیص و جداسازی این باکتری می‌باشد. 

کلیدواژه‌ها


عنوان مقاله [English]

Detection and isolation of Mycoplasma genitallium in evaporated urine from pregnant women by PCR method

نویسندگان [English]

  • Monireh Rahimkhani 1
  • Alireza Mordadi 2
1 Associate professor, Department of Lab Medical Sciences, School of Allied Medical Sciences, Tehran University of Medical Sciences, Tehran, Iran.
2 M.Sc. of Bacteriology, Department of Epidemiology, Pasteur Institute, Tehran, Iran.
چکیده [English]

Introduction: Mycoplasma is one of the most important bacterial causes of genital infections in women and men, followed by premature birth and even abortion in pregnant women. The present study was performed aimed to detect and isolate Mycoplasma genitallium in some pregnant women in first trimester of pregnancy.
Methods: In this cross-sectional (descriptive-analytical) study which was performed in 2020-2021, evaporated urine sampling was collected from 66 pregnant women who were referred to the health center for screening tests on first trimester of pregnancy (between 11-14 weeks). In this way, these women did not urinate for at least 2 hours and then urine samples were collected in sterile containers. Urine sediment DNA was extracted and the genome of Mycoplasma was detected by PCR method. Data were analyzed by SPSS software (version 20) and Independent T-test. P<0.05 was considered statistically significant.
Results: Mycoplasma genitallium genome was isolated from 17.65 % of evaporated urine sediment samples by PCR method.
Conclusion: For isolation and detection of Mycoplasma in pregnant women, sampling of vaginal or cervix discharge have some dangers, thus evaporated urine sampling is a safer and more reliable method.

کلیدواژه‌ها [English]

  • Evaporated urine
  • Mycoplasma
  • PCR
  • Pregnant women
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