عنوان مقاله [English]
Introduction: Trichomonas vaginalis is a parasite from flagellate which leads to trichomomal vaginitis. Also, it can be responsible for various complications such as discharge, painful urination, genital irritation, discomfort after intercourse, premature rupture of membranes, and preterm labor. Metronidazole is a selected drug for treatment of the disease that carcinogenesis effects of this drug and also resistance of this parasite toward metronidazole have been reported in recent years. Since physalis alkekengi plant has been applied for treatment of gastrointestinal diseases, hepatitis, viral, gonorrhea, gout, diabetes, and sore throat, therefore, this study was performed with aim to determine in vitro effects of aqueous and alcoholic extracts of physalis alkekengi on Trichomonas vaginalis.
Methods: This … study was performed in 2015 to assess the anti-parasite effects of physalis alkekengi on Trichomonas vaginalis. At first, the aqueous and alcoholic extracts were prepared and proliferation of parasites in TYM Diamond culture medium was performed. The concentrations of extract were: 8000, 4000, 2000, 1000, 500 and 250 µg/ml. The proliferation was evaluated at 24, 48 and 72 hours compared to control (metronidazole 50 µg/ml) group. In all of cases, the number of live and dead parasites was counted with Trypan blue and Neubauer slide. Data was analyzed by software SPSS (version 18) and one-way variance analysis. PResults: The aqueous and alcoholic extracts had significant inhibitory effects on Trichomonas vaginalis parasite proliferation (P<0.01). %IC50 aqueous and alcoholic extracts of physalis alkekengi at 24 h in concentration of 8000 and 4000 µg/ml were 100% compared to the control group (metronidazole). Alcoholic extract of the physalis alkekengi plant showed 100% inhibitory effect in concentration of 2000 µg/ml at 48 hours after exposure to the parasite. While, the aqueous extract in the same time at 8000µg/ml was effective.
Conclusion: After evaluation of effectiveness mechanism, the aqueous and alcoholic extracts of the physalis alkekengi plant can be alternative treatment potential in cases of resistance to metronidazole.