بررسی اثر عصاره هیدروالکلی کلم قرمز بر مهار رشد و القاء آپوپتوز در سلول های سرطانی پستان (MCF-7)

نوع مقاله: اصیل پژوهشی

نویسندگان

1 دستیار گروه فارماکولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.

2 استادیار مرکز تحقیقات فارماکولوژیک گیاهان دارویی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.

3 دانشیار گروه فارماکولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.

چکیده

مقدمه: سبزیجات براسیکا متعلق به خانواده کلم بوده و شامل انواع مختلف کلم (قرمز، سبز و ...)، گل کلم، کلم بروکلی و کلم پیچ می باشد. گزارشات نشان می دهند که مصرف این گیاهان در کاهش خطر ابتلاء به بیماری های حاد مانند بیماری قلبی عروقی و سرطان مؤثرند. یک گروه از این خانواده کلم قرمز می باشد. مطالعات نشان داده اند که کلم قرمز حاوی ترکیباتی است که در درمان بیماری های مختلف از جمله سرطان نقش دارند. مطالعه حاضر با هدف بررسی اثرات سایتوکسیک و آپاپتوتیک عصاره هیدروالکلی کلم قرمز بر رده سرطانی پستان (MCF-7) انجام شد.
روش کار: مطالعه حاضر اواخر سال 1392 به صورت برون تنی بر روی سلول های سرطانی پستان (MCF-7) در مرکز تحقیقات فارماکولوژیک گیاهان دارویی انجام گردید. سلول ها در محیط کشت DMEM با 10% سرم جنینی گاو، 100 واحد در میلی لیتر پنی سیلین و 100 میکروگرم در میلی لیتر استرپتومایسین کشت شده و با غلظت های متفاوتی از عصاره کلم قرمز انکوبه شدند. میزان زنده ماندن سلول ها با تست MTT ارزیابی شد. میزان سلول های آپوپتوتیک نیز با استفاده از رنگ آمیزی پروپیدیوم یداید (PI) به وسیله فلوسایتومتری (پیک SUB-G1) اندازه گیری شد.
یافته ها: کلم قرمز رشد سلول های سرطانی را به صورت وابسته به دوز و زمان مهار کرد. بعد از 24 ساعت غلظت 2000 میکروگرم در میلی لیتر (05/0>p)، بعد از 48 ساعت غلظت های 1000 (05/0>p)، 1500 (05/0>p) و 2000 (01/0>p) و پس از گذشت 72 ساعت غلظت های 500 (05/0>p)، 1000 (01/0>p)، 1500 (01/0>p) و 2000 (001/0>p) به صورت معنی داری مرگ و میر سلول های زنده را افزایش دادند. همچنین عصاره در غلظت های 1000 (05/0>p)، 1500 (01/0>p) و 2000 (001/0>p) باعث تحریک آپپتوز شد. همچنین تست MTT نشان داد که عصاره سمیتی بر سلول های نرمال در زمان های 24، 48 و 72 ساعت نداشت. میزان غلظتی از عصاره که بتواند رشد سلول ها را 50 درصد مهار کند، (IC50) 2685 میکروگرم بر میلی لیتر می باشد.
نتیجه گیری: کلم قرمز از طریق تحریک آپوپتوز میزان مرگ و میر سلول های MCF-7 را افزایش می دهد، در نتیجه کلم قرمز می تواند در درمان سرطان پستان مؤثر باشد.

کلیدواژه‌ها


عنوان مقاله [English]

The Evaluation of the Effects of Hydro-Alcoholic Extract of Brassica oleracea (Red Cabbage) on Growth Inhibition and Apoptosis Induction in Breast Cancer Cell Line MCF-7

نویسندگان [English]

  • Davood Mahdian 1
  • Azar Hosseini 2
  • Seyed Hadi Mousavi 3
  • Mahdi Bihamta 1
  • Mohamad Mahdi Vahedi 1
1 Assistant, Department of Pharmacology, School of Medicine, University of Medical Sciences, Mashhad, Iran.
2 Assistant Professor, Pharmacological Research Center of Medicinal Plants, Faculty of Medicine, University of Medical Sciences, Mashhad, Iran.
3 Associate Professor, Department of Pharmacology, School of Medicine, University of Medical Sciences, Mashhad, Iran.
چکیده [English]

Introduction: Brassica vegetables belong to Cruciferous family, and include diverse types of cabbage (green, red, etc.), cauliflower, broccoli, Brussels sprouts and kale. Several studies have shown that consumption of these vegetables can lower incidence rate of cardiovascular and cancer diseases. One of these vegetables is Brassica oleracea. Studies suggested that red cabbage contains beneficial compounds which play a significant role in treatment of different disease such as cancer. This study evaluated cytotoxic and pro-apoptotic effects of hydro-alcoholic extract of Brassica oleracea on cell line MCF-7.
Methods: The in-vitro study was carried out on breast cancer cells (MCF-7) at the end of the year 2014 in Pharmacological Research Center of Medicinal Plants. Cells were cultivated in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin and 100 µg/ml streptomycin. Then, the cells were incubated with different concentrations of extracts. Cell viability was assessed by dint of MTT assay. Apoptotic cells were detected using propidium iodide (PI) staining of DNA fragments by flow cytometry (sub-G1 peak).
Results: RC[S1]  inhibited the growth of malignant cells in a time-and dose-dependent manner. Following 24 hours, extract undermined cell viability at dose of 2000 µg/ml (P<0.05), after 48 hours increased cell death at doses of 1000 (P<0.05), 1500 (P<0.05) and 2000 (P<0.01). After 72h, rate of cell death increased at doses of 500 (P<0.05), 1000 (P<0.01), 1500 (P<0.01) and 2000 (P<0.001). Also, the extract induced apoptosis at doses of 1000 (P<0.05), 1500 (P<0.01) and 2000 (P<0.001). Further, the extract did not have any cytotoxicity effect on normal cells after 24, 48 and 72 hours. The IC50 was 2685 µg/ml.
Conclusion: The results of this study indicated that red cabbage accelerated cell death in MCF-7 cells via apoptosis induction. Therefore, red cabbage can be considered an effective treatment for breast cancer.

کلیدواژه‌ها [English]

  • Red Cabbage
  • Breast Cancer
  • Apoptosis
  • MCF-7
  • Cell death
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