بررسی بهبود پارامترهای اسپرم انسان بعد از آماده سازی به روش Swim up

نوع مقاله: اصیل پژوهشی

نویسندگان

1 دانشیارزنان وزایمان،دانشکده پزشکی دانشگاهعلومپزشکی بابل

2 استادیارآناتومی و جنین شناسی،دانشکده پزشکی دانشگاهعلومپزشکی بابل

چکیده

مقدمه: حدود10 الی15 درصد زوجها دارای مشکل ناباروری اند و برای آنهایی که به درمان دارویی پاسخ نمی دهند
استفاده ازروشهای کمک باروری، اجتناب ناپذیر است. swim up یکی از روشهایی است که باعث بهبود کیفیت
اسپرم میشود. این مطالعه با هدف بررسی تاثیر swim up بر حرکت، غلظت و مورفولوژی اسپرم انجام پذیرفت.
روش کار: این مطالعه تحلیلی- آزمایشگاهی، بر روی نمونههای طبیعی مایع منی 30 مرد مراجعه کننده به مرکز
ناباروری فاطمه الزهراء (س) دانشگاه علوم پزشکی بابل کاندید لقاح خارج رحمی انجام شد. قبل و بعد از swim up
اطلاعات لازم شامل تعداد، حرکت و مورفولوژی اسپرم ها ثبت گردید. اطلاعات توسط نرمافزار آماری SPSS نسخه
10 و آزمون تی تست زوج آنالیز شد.
× 64/1) بطور معنیداری کمتر از قبل از 6 swim up نتایج: میانگین تعداد اسپرمها بعد از 10 ) swim up
10 )
6 .((p<0/001) بود swim up از بعد %85/8 و swim up از قبل %60/3 اسپرم حرکت میانگین .(p<0/001) بود (87/3×
اسپرم III ،II ،I گرید در (p=0/003) درصد 21/6 و (p<0/001) 12/6 ،(p=0/083) 2/5 بهبود سبب ترتیب به swim up
شده بود. میانگین درصد مورفولوژی طبیعی قبل و بعد از swim up به ترتیب 11/2% و 16/3% بود (p<0/001).
نتیجهگیری: بعد از آماده سازی به روش swim up، میزان مورفولوژی طبیعی، حرکت و گریدینگ اسپرم به نحو
مطلوبی افزایش می یابد. 

کلیدواژه‌ها


عنوان مقاله [English]

The Effects of Swim up Method on Sperm Parameters

نویسندگان [English]

  • Zahra Basirat 1
  • Seyed Gholamali Jour Sarayee 2
1 Associate Professor of Obstetrics & Gynecology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
2 Assistant Professor of Anatomy, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
چکیده [English]

Introduction: Approximately, 10-15% of couples suffer from infertility. Various therapeutic approaches have been used to treat infertility; however for those who do not response to treatment using some methods to assist fertility are inevitable. Swim up is one of these methods that enhance the sperm quality. This aim of study was to determine the effects of swim up method on motility, density and morphology of sperms.
 
Methods and Material: In this experimental-analytical study the semen specimens of 30 individuals with normal parameters (for selection of sperm with better quality) referred to Fateme-Alzahra Infertility Center affiliated to Babol University of Medical Sciences for performing in-vitro fertilization were used. The men with cigarette smoking or addiction excluded. Pre-and post swim-up data including the count, motility, morphology, and grading of sperms were recorded. The data were analyzed using SPSS version 10. Paired t test was used for analysis.
 
Results: The mean sperm count after swim up (64.1×106) was significantly (p<0.001) lower than before the swim up (87.3×106). Mean sperm motility was 60.3% before swim up and 85.8% after swim up (p<0.001). By using swim up method 2.5% (p=0.083), 12.6% (p<0.001) and 21.6٪ (p=0.003) improvement was seen in sperm grade I , II and III respectively .The mean percentage of sperms with normal morphology before and after the swim-up was 11.2% and 16.3%, respectively (p<0.001).
 
Conclusion: Preparation of sperms by swim-up method improves the normal sperm morphology, motility, and grading.

کلیدواژه‌ها [English]

  • Swim up Method
  • Sperm Count
  • Morphology
  • Motility
  • Grading
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