بررسی فراوانی ویروس ابشتین- بار در نمونه‌های بافتی زنان مبتلا به سرطان پستان در شهر مشهد

نوع مقاله : اصیل پژوهشی

نویسندگان

1 رزیدنت بیماری‌های داخلی، دپارتمان بیماری‌های داخلی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.

2 استادیار خون و سرطان بالغین، دپارتمان بیماری‌های داخلی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.

3 دانشجوی پزشکی، کمیته تحقیقات دانشجویی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.

4 استادیار پزشکی مولکولی، مرکز تحقیقات پاتولوژی مولکولی سرطان، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.

5 استادیار بیماری‌های داخلی، دپارتمان بیماری‌های داخلی، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران.

10.22038/ijogi.2024.77354.5965

چکیده

مقدمه: سرطان پستان، یکی از شایع‌ترین سرطان ها در جهان است که در دهه اخیر افزایش چشم‌گیری در میزان بروز آن رخ داده است. اگرچه عوامل خطر و علل سرطان پستان به‌طور کامل شناخته نشده است، اما مطالعاتی در مورد ارتباط بین سرطان پستان و عوامل ویروسی از جمله ویروس اپشتین- بار (EBV) گزارش شده است، لذا مطالعه حاضر با هدف بررسی ارتباط بین EBV و سرطان پستان انجام شد.
روش‌کار: در این مطالعه مقطعی که بر روی نمونه­ های پاتولوژی بافت پستان بین سال­ های 1401-۱۳۹۶ در بیمارستان‌های مشهد صورت گرفت، ۳۰ نمونه پارافینه از بیماران مبتلا به سرطان پستان و ۳۳ نمونه پارافینه به‌عنوان گروه کنترل از بیماری­ های خوش‌خیم پستان جمع‌آوری شد. بعد از فرآیندهای استخراج DNA، بررسی از نظر ویروس EBV با روش واکنش زنجیره‌ای پلیمراز (PCR) انجام گرفت. تجزیه و تحلیل داده ­ها با استفاده از نرم‌افزار آماری SPSS (نسخه ۲۰) و آزمون‌های کای دو، دقیق فیشر و تی تست انجام شد. میزانp  کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته ­ها: بر اساس نتایج به‌دست آمده، از PCR ۶۳ نمونه بیوپسی بافتی انجام شده، هیچ‌یک از نمونه ­های گروه بدخیم و کنترل از نظر EBV مثبت نبودند.
نتیجه ­گیری: حضور ژن ویروس EBV در یک گروه قابل توجه از زنان مبتلا به سرطان پستان در شهر مشهد تأیید نشد. با این‌حال مطالعات بیشتر با حجم نمونه بزرگ‌تر برای اعتبارسنجی نتایج ضرورت دارد.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Epstein-Barr virus (EBV) frequency in tissue samples of women with breast cancer in Mashhad, Iran

نویسندگان [English]

  • Ahmad Tafaghod 1
  • Samaneh Sajjadi 2
  • Mohammadhossein Taherynejad 3
  • Hassan Mehrad- Majd 4
  • Sahar Ravanshad 5
1 Resident, Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
2 Assistant professor of Blood and Cancer of Adult, Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3 Medical Student, Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
4 Assistant Professor of Molecular Medicine, Cancer Molecular Pathology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
5 Assistant professor, Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
چکیده [English]

Introduction: Breast cancer is one of the most common cancers in the world that its incidence rate has increased over the last decade. Although the causes and risk factors of breast cancer are not fully known, studies have been reported on the relationship between breast cancer and viral agents, including the Epstein-Barr virus (EBV). Therefore, the present study was performed with aim to explore the correlation between EBV and breast cancer.
Methods: In this cross-sectional study which was conducted using breast tissue pathology samples collected from Mashhad hospitals between 2017 and 2022, 30 paraffin tissue samples from breast cancer patients and 33 paraffin tissue samples as a control group from benign breast diseases were collected. After the DNA extraction processes, the EBV virus was tested by polymerase chain reaction (PCR). Data analysis was done using SPSS software (version 20) and Chi-square, Fisher's exact and t-test. P<0.05 was considered statistically significant.
Results: According to the PCR results of 63 tissue biopsy samples, none of the malignant and control group samples were EBV-positive.
Conclusion: The presence of the EBV virus gene was not confirmed in a group of women with breast cancer in Mashhad. However, further studies with a larger sample size are needed to validate the results.

کلیدواژه‌ها [English]

  • Breast Cancer
  • Epstein-Barr Virus
  • Polymerase Chain Reaction

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مجله زنان، مامایی و نازایی ایران
دوره 26، شماره 12
اسفند 1402
صفحه 30-38

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