بررسی میزان بیان miR200a به‌عنوان یک بیومارکر در زنان مبتلا به زایمان زودرس

نوع مقاله: اصیل پژوهشی

نویسندگان

1 کارشناس ارشد گروه ژنتیک، دانشکده علوم و فناوری‌های نوین، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران.

2 استادیار گروه زنان و مامایی، فلوشیپ پریناتولوژی گروه زنان و زایمان، دانشکده پزشکی، دانشگاه علوم پزشکی اردبیل، اردبیل، ایران.

3 استادیار گروه ژنتیک، دانشکده علوم و فناوری‌های نوین، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران.

4 دانشیار گروه ژنتیک پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی اردبیل، اردبیل، ایران

چکیده

مقدمه: زایمان زودرس، یکی از مشکلات اصلی بهداشتی محسوب می‌شود و بعد از ناهنجاری‌های مادرزادی، اصلی‌ترین عامل ابتلاء به بیماری و مرگ‌ومیر نوزادان است. با توجه به نقش گسترده MicroRNA‌ها به‌عنوان بیومارکر در تشخیص انواع بیماری‌ها، مطالعه حاضر با هدف بررسی میزان بیان miR200a به‌عنوان یک بیومارکر در زنان مبتلا به زایمان زودرس در استان اردبیل انجام شد.
روش‌کار: این مطالعه مورد شاهدی از مهر 1396 تا آذر 1397 بر روی 50 زن باردار نخست باردار سالم و 50 زن نخست باردار که برای زایمان زودرس به بیمارستان علوی شهر اردبیل مراجعه کرده بودند، انجام شد. پس از استخراج MicroRNA از خون و سنتز cDNA به‌منظور بررسی میزان بیان miR200a از تکنیک Real Time PCR استفاده شد. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS (نسخه 20) و آزمون آنوا، وان وی و تی تست انجام شد. میزان p کمتر از 05/0 معنادار در نظر گرفته شد.
یافته‌ها: میزان بیان miR200a در گروه نرمال 04/0±35/0 و در گروه زایمان زودرس 09/0±24/0 بود که نسبت به گروه نرمال بارداری، به میزان 11% کاهش بیان داشت. همچنین اختصاصیت و حساسیت انجام این تست برای بررسی زایمان زودرس به‌ترتیب برابر 4/53% و 74% بود که توسط منحنی ROC مورد بررسی قرار گرفت. این نتایج از نظر آماری معنادار بود (05/0≥p).
نتیجه‌گیری: در این پژوهش میزان بیان microRNA200a مورد مطالعه در بین زنان با زایمان زودرس و طبیعی متفاوت بود و به‌نظر می‌رسد، بتوان از miR200a به‌عنوان یک بیومارکر جدید برای پیش‌بینی زایمان زودرس استفاده کرد.

کلیدواژه‌ها


عنوان مقاله [English]

Expression of miR200a as a biomarker in women with preterm delivery

نویسندگان [English]

  • Effat Seyed Hashemi 1
  • Roghaye Dargahi 2
  • Malihe Entezari 3
  • Seyed Saeed l Hosseini As 4
1 M.Sc. of Genetics, School of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
2 Assistant professor, Department of Obstetrics and Gynecology, Fellowship of Perinatology, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
3 Assistant professor, Department of Genetics, School of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
4 Associate professor, Department of Medical Genetics, Faculty of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
چکیده [English]

Introduction: Preterm delivery is one of the main health problems and after congenital anomalies is the main cause of neonatal disease and mortality. Considering the main role of microRNAs as Biomarkers in the diagnosis of various types of diseases, this study was performed with aim to investigate the expression of miR200a as a biomarker in women with preterm delivery in Ardabil province.
Methods: This case-control study was performed on 50 healthy nulliparous women and 50 nulliparous women who had referred to Ardabil Alavi hospital for preterm labor from October 2017 to December 2018. After extracting MicroRNA and synthesis of cDNA, real time PCR technique was used to determine miR200a expression. Data were analyzed by SPSS software (version 20) and ANOVA, t-test, and v-one test. P<0.05 was considered statistically significant.
Results: The expression of miR200a in the normal group was 0.35±0.04, and in the preterm delivery group was 0.24± 0.09 that had 11% decreases in expression compared to the healthy group. Also, specificity and sensitivity of this test for evaluation of preterm delivery was 53.4% and 74%, respectively, which was assessed by ROC curve. These results were statistically significant (P ≤ 0.05).
Conclusion: The expression of microRNA200a as a Biomarker was different among normal pregnant women and those with preterm delivery; it seems that microRNA200a can be used as a new marker to predict preterm delivery.

کلیدواژه‌ها [English]

  • Biomarker
  • MicroRNA
  • Preterm delivery
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