تأثیر یک جلسه تمرین ورزشی فزاینده پس از مصرف پروتئین وی بر MFO، Fatmax و مقاومت به انسولین زنان دارای اضافه وزن

نوع مقاله: اصیل پژوهشی

نویسندگان

1 کارشناس ارشد فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران.

2 استاد گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران.

چکیده

مقدمه: پژوهش­های اخیر نشان داده‌اند که افزایش سهم پروتئین رژیم غذایی می­تواند به کاهش معنادار وزن بدن و توده چربی افراد دارای اضافه وزن کمک کند. مطالعه حاضر با هدف بررسی تأثیر یک جلسه فعالیت ورزشی فزاینده پس از دو شیوه مکمل­گیری پروتئین وی بر MFO، Fatmax و مقاومت به انسولین زنان دارای اضافه وزن انجام شد.
روش کار: این مطالعه کارآزمایی بالینی، با طرح پیش و پس آزمون روی یک گروه تجربی (15 زن دارای اضافه وزن) در سال 1394 در شهرستان مشهد انجام شد. در این مطالعه به فاصله 10-7 روز Fatmax، MFO و شاخص مقاومت به انسولین زنان در سه مرحله: بدون مصرف پروتئین وی و به ترتیب 30 و 75 دقیقه پس از بارگیری پروتئین وی طی یک جلسه تمرین فزاینده تا واماندگی به روش الایزا اندازه­گیری شد. تجزیه و تحلیل داده­ها با استفاده از نرم‌افزار آماری SPSS (ویرایش 16) و آزمون تحلیل واریانس انجام شد. میزان p کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته­ها: میزان MFO و Fatmax بین سه مرحله تفاوت معنی­داری نداشت (05/0<p). مقادیر مقاومت به انسولین در سه مرحله در مقایسه با حالت ناشتا معنادار بود (05/0>p)، اما بیشترین میزان مقاومت به انسولین مربوط به پایان مرحله دوم بود.
نتیجه‌گیری: مصرف مکمل پروتئینی وی 30 و 75 دقیقه پیش از ورزش فزاینده، تأثیری بر میزان MFO و Fatmax ندارد، اما موجب افزایش مقاومت به انسولین می­شود.

کلیدواژه‌ها


عنوان مقاله [English]

Effects of One Session of Progressive Training after Whey Protein Consumption on the MFO, Fatmax, and Insulin Resistance in Overweight Women

نویسندگان [English]

  • Atefeh Seyedi 1
  • Seyed Reza Attarzadeh Hosseini 2
1 M.Sc. in Sport Physiology, School of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
2 Professor, Department of Sport Physiology, School of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran.
چکیده [English]

Introduction: Recent findings show that increased dietary protein intake could significantly reduce the body weight and fat mass in overweight individuals. The present study aimed to investigate the effects of one session of progressive training after two modes of whey protein supplementation on the maximal fat oxidation (MFO), Fatmax, and insulin resistance in overweight women.
Methods: This clinical trial study was conducted with a pretest-posttest design on an experimental group consisting of 15 overweight women in Mashhad, Iran in 2016. At 7-10 day intervals, Fatmax, MFO, and insulin resistance index were measured in three stages of no whey protein consumption and at 30 and 75 minutes after whey protein loading during one session of progressive training until failure. Data collection tool was the ELISA kit. Data analysis was performed in SPSS version 16.0 using the analysis of variance (ANOVA), and P-value of less than 0.05 was considered significant.
Results: No significant differences were observed in the MFO and Fatmax in the three stages of the study (P>0.05). However, the insulin resistance index was significant in the three stages compared to the fasting state (P<0.05), and the highest rate of insulin resistance was noted at the end of the second stage.
Conclusion: According to the results, whey protein supplementation at 30 and 75 minutes before progressive training has no effect on the MFO and Fatmax, while it could increase insulin resistance.

کلیدواژه‌ها [English]

  • MFO
  • Fatmax
  • Whey Protein
  • Overweight
  1. Gholami Khujin R. Treatment of obesity with surgery. New Med 2011; 515:484-91. (Persian).
  2. Karandish M, Tabesh H, Kabli E. Contributing factors of treatment drop-out in overweight or obese patients in Ahvaz. Jentashapir J Health Res 2013; 4(2):111-20. (Persian).
  3. Tofighi A. The effects of a selected aerobic exercise along with a controlled diet on weight loss in obese men. Iran J Nutr Sci Food Technol 2014; 9(2):85-94.
  4. Meckling KA, Sherfey R. A randomized trial of a hypocaloric high-protein diet, with and without exercise, on weight loss, fitness, and markers of the Metabolic Syndrome in overweight and obese women. Appl Physiol Nutr Metab 2007; 32(4):743-52.
  5. Deibert P, König D, Schmidt-Trucksaess A, Zaenker K, Frey I, Landmann U, et al. Weight loss without losing muscle mass in pre-obese and obese subjects induced by a high-soy-protein diet. Int J Obes Metab Disord 2004; 28(10):1349-52.
  6. Skov A, Toubro S, Rønn B, Holm L, Astrup A. Randomized trial on protein vs carbohydrate in ad libitum fat reduced diet for the treatment of obesity. Int J Obes Metab Disord 1999; 23(5):528-36.
  7. Baer DJ, Stote KS, Paul DR, Harris GK, Rumpler WV, Clevidence BA. Whey protein but not soy protein supplementation alters body weight and composition in free-living overweight and obese adults. J Nutr 2011; 141(8):1489-94.
  8. Davati N. The role of functional proteins in whey. Second National Conference on functional food (operator), Tehran, Iran; 2009. P. 277-85. (Persian).
  9. Ahmadi Kani Golzar F, Sheikholeslami Vatani D, Kashkooli V, Moradi H, Farhangian M. The effects of whey protein isolate supplement and strength training on weight loss, body composition, strength and muscle hypertrophy in overweight young men. Iran J Nutr Sci Food Technol 2012; 7(2):37-46. (Persian.(
  10. Morifuji M, Sakai K, Sanbongi C, Sugiura K. Dietary whey protein downregulates fatty acid synthesis in the liver, but upregulates it in skeletal muscle of exercise-trained rats. Nutrition 2005; 21(10):1052-8.
  11. Bouthegourd JC, Roseau SM, Makarios-Lahham L, Leruyet PM, Tomé DG, Even PC. A preexercise α-lactalbumin-enriched whey protein meal preserves lipid oxidation and decreases adiposity in rats. Am J Physiol Endocrinol Metab 2002; 283(3):E565-72.
  12. Ha E, Zemel MB. Functional properties of whey, whey components, and essential amino acids: mechanisms underlying health benefits for active people (review). J Nutr Biochem 2003; 14(5):251-8.
  13. Blomstrand E, Eliasson J, Karlsson HK, Köhnke R. Branched-chain amino acids activate key enzymes in protein synthesis after physical exercise. J Nutr 2006; 136(1):269S-73S.
  14. Blomstrand E, Hassmen P, Ekblom B, Newsholme EA. Administration of branched-chain amino acids during sustained exercise—effects on performance and on plasma concentration of some amino acids. Eur J Appl Physiol Occup Physiol 1991; 63(2):83-8.
  15. Venables MC, Achten J, Jeukendrup AE. Determinants of fat oxidation during exercise in healthy men and women: a cross-sectional study. J Appl Physiol 2005; 98(1):160-7.
  16. Safari MS, Mohebbi H, Damirchi A, Hovanlo F. Effect of reduced muscle glycogen on MFO and Fatmax during exercise in untrained men. J Metab Exer 2013; 2(2):113-23. (Persian).
  17. Farnfield MM, Trenerry C, Carey KA, Cameron-Smith D. Plasma amino acid response after ingestion of different whey protein fractions. Int J Food Sci Nutr 2008; 60(6):476-86.
  18. Jeukendrup AE, Wallis GA. Measurement of substrate oxidation during exercise by means of gas exchange measurements. Int J Sports Med 2005; 26(Suppl 1):S28-37.
  19. Sâmpelean D, Hanescu B, Han A, Adam M, Casoinic F. The Prognosis of Glycoregulation Disturbances and Insulin Secretion in Alcoholic and C Virus Liver Cirrhosis. Rom J Intern Med. 2009;47(4):387-92.
  20. Marzouki H, Gmada N, Farhani Z, Hssin N, Shephard R, Bouhlel E. Crossover and maximal fat oxidation points during running and cycling in sedentary subjects. Sci Sports 2015; 30(4):196-203.
  21. Mohebbi H, F R. The effect of type of exercise on fat oxidation, MFO and FAtmax in young women. Olympic. 2009;3:139-49(Persian).
  22. Newsholme EA, Blomstrand E. Branched-chain amino acids and central fatigue. J Nutr 2006; 136(1 Suppl):274S-6S
  23. Achten J, Gleeson M, Jeukendrup AE. Determination of the exercise intensity that elicits maximal fat oxidation. Med Sci Sports Exerc 2002; 34(1):92-7.
  24. Achten J, Venables MC, Jeukendrup AE. Fat oxidation rates are higher during running compared with cycling over a wide range of intensities. Metab Clin Exper 2003; 52(6):747-52.
  25. Romijn JA, Coyle EF, Sidossis LS, Gastaldelli A, Horowitz JF, Endert E, et al. Regulation of endogenous fat and carbohydrate metabolism in relation to exercise intensity and duration. Am J Physiol 1993; 265(3 Pt 1):E380-91.
  26. Maughan R, Gleeson M, Greenhaff PL, editors. Biochemistry of Exercise and Training 1951.
  27. Frid AH, Nilsson M, Holst JJ, Björck IM. Effect of whey on blood glucose and insulin responses to composite breakfast and lunch meals in type 2 diabetic subjects. Am J Clin Nutr 2005; 82(1):69-75.
  28. Pal S, Ellis V. The acute effects of four protein meals on insulin, glucose, appetite and energy intake in lean men. Br J Nutr 2010; 104(8):1241-8.
  29. Klupa T, Benbenek-Klupa T, Matejko B, Mrozinska S, Malecki MT. The impact of a pure protein load on the glucose levels in type 1 diabetes patients treated with insulin pumps. International journal of endocrinology. 2015;2015.
  30. Frid AH, Nilsson M, Holst JJ, Björck IM. Effect of whey on blood glucose and insulin responses to composite breakfast and lunch meals in type 2 diabetic subjects–. The American Journal of Clinical Nutrition. 2005;82(1):69-75.
  31. Newsholme P, Brennan L, Bender K. Amino acid metabolism, β-cell function, and diabetes. Diabetes 2006; 55(Suppl 2):S39-47.
  32. Zhou Y, Jetton TL, Goshorn S, Lynch CJ, She P. Transamination is required for α-ketoisocaproate but not leucine to stimulate insulin secretion. J Biol Chem 2010; 285(44):33718-26.
  33. Honardoost M, Sarookhani M, Arefian E. Molecular mechanism of insulin resistance. J Qazvin Univ Med Sci 2014; 18(5):57-64. (Persian).
  34. Zeng G, Nystrom FH, Ravichandran LV, Cong LN, Kirby M, Mostowski H, et al. Roles for insulin receptor, PI3-kinase, and Akt in insulin-signaling pathways related to production of nitric oxide in human vascular endothelial cells. Circulation 2000; 101(13):1539-45.
Bhattacharya S, Dey D, Roy SS. Molecular mechanism of insulin resistance. J Biosci 2007; 32(2):405-13.