بررسی مقایسه روشهای کشت و PCR در تعیین شیوع مایکوپلاسما هومینس نمونههای اندوسرویکس بیماران مراجعه کننده به درمانگاه ناباروری بیمارستان فاطمیه همدان در سال 1395

نوع مقاله: اصیل پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد میکروب‌شناسی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران.

2 Ph.D باکتری‌شناسی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران.

3 متخصص زنان و زایمان، دانشکده پرستاری و مامایی، دانشگاه علوم پزشکی همدان، همدان، ایران

4 دانشجوی Ph.D باکتری‌شناسی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی همدان، همدان، ایران.

چکیده

مقدمه: باکتری مایکوپلاسما هومینیس به‌طور همزیست در دستگاه تناسلی زنان زندگی می‌کند. بررسی­های انجام شده در سال­های اخیر نشان می­دهد که حضور این باکتری مرتبط با اختلالاتی از قبیل واژینوز، ناباروری، سقط و زایمان زودرس می­باشد. مطالعه حاضر با هدف بررسی فراوانی مایکوپلاسما هومینیس در زنان مراجعه کننده به درمانگاه ناباروری بیمارستان فاطمیه همدان انجام شد.
روش‌کار: این مطالعه اپیدمیولوژیک - توصیفی در سال 1395 بر روی 234 نفر از بیماران مراجعه کننده به درمانگاه نازایی بیمارستان زنان فاطمیه شهر همدان انجام گرفت. با استفاده از سواب، از اندوسرویکس 234 بیمار زن که حداقل یکی از علائم واژینوز، ناباروری، سقط و زایمان زودرس را داشتند، نمونه‌برداری انجام شد. از محیط کشت با حذف مرحله فیلتراسیون و از تکنیک مولکولی PCR برای ردیابی ژن 16s rRNA به‌عنوان دو روش تشخیص باکتری استفاده شد. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS و آزمون مک نمار انجام شد. میزان p‌ کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته‌ها: فراوانی مایکوپلاسما هومینیس در هر دو روش، 7/13% به‌دست آمد. ضریب همبستگی بین روش‌های کشت و  PCRبرای تشخیص این باکتری نسبتاً مطلوب بود (5/0=k). تعداد 41 نفر (4/24%) از بیماران با سابقه حداقل یک بار سقط جنین و 148 نفر (2/14%) از بیماران دارای واژینوز، از نظر مایکوپلاسما آگار مثبت تشخیص داده شدند. بیشترین شیوع مایکوپلاسما هومینیس در محدوده سنی 39-30 سال مشاهده شد.
نتیجه‌گیری: اگرچه تکنیک مولکولی PCR حساسیت بالایی در تشخیص مایکوپلاسما هومینیس دارد، ولی روش کشت تغییر یافته برای تشخیص این باکتری از حساسیت قابل قبولی برخوردار می‌باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Comparison of PCR and culture methods to determine the prevalence of Mycoplasma hominis in woman's endocervical samples referred to Infertility Center of Hamadan Fatemieh Hospital in 2016

نویسندگان [English]

  • Farzaneh Moradi 1
  • Rasoul Yousefi Mashouf 2
  • Mohammad Yousef Alikhani 2
  • Soghra Rabiei 3
  • Hamideh Parsapour 3
  • Saman Saadat 4
  • Jalal Ghaderkhani 4
1 M.Sc. Student of Medical Microbiology, Faculty of Medicine, Hamedan University of Medical Sciences, Hamadan, Iran.
2 Ph.D. of Medical Bacteriology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
3 Gynecologist, Faculty of Nursing and Midwifery, Hamadan University of Medical Sciences, Hamadan, Iran.
4 Ph.D. Student of Medical Bacteriology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
چکیده [English]

Introduction: The Mycoplasma Hominis bacterium lives on in the female reproductive system. Recent studies have shown that the presence of this bacterium is associated with disorders such as vaginosis, infertility, abortion and preterm labor. This study was performed with aim to determine the frequency of Mycoplasma hominis in women referred to Infertility Clinic of Hamadan Fatemieh Hospital.
Methods: This epidemiologic-descriptive study was performed on 234 patients referred to the infertility clinic of Hamadan Fatemieh Hospital in 2016. Screening was performed using swabs from the endocervix of 234 female patients who had at least one of the symptoms of vaginosis, infertility, abortion and preterm labor. The culture medium was removed by filtration and the PCR molecular technique was used to trace the 16s rRNA gene as two methods for detection of bacteria. Data was analyzed by SPSS software and McNemar's test. P<0.05 was considered significant.
Results: The frequency of Mycoplasma hominis in both methods was 13.7%. The correlation coefficient between culture and PCR methods was relatively desirable for detection of this bacterium (k=0.5). 41 patients (24.4%) with history of at least one abortion and 148 (14.2%) with vaginosis were diagnosed as positive Mycoplasma agar. The highest prevalence of Mycoplasma hominis was observed in the age range of 30-39 years.
Conclusion: Although the PCR molecular technique has high sensitivity to diagnose Mycoplasma hominis, but the modified culture method has an acceptable sensitivity to detect this bacterium.

کلیدواژه‌ها [English]

  • Abortion
  • Culture
  • Infertility
  • Mycoplasma hominis
  • PCR
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