Effects of Human Menopausal Gonadotropin and Recombinant Follicle Stimulating Hormones on In Vitro Maturation of Human Immature Oocytes and Rate of Eight-Cell Embryos Mediated by Intracytoplasmic Sperm Injection

Document Type : Original Article

Authors

1 Assistant Professor of Biology, Faculty of Sciences, Islamiz Azad University, Mashhad, Iran

2 Assistant Professor, Montaserieh Research & Clinical Center for Infertility, Mashhad University of Medical Sciences, Mashhad, Iran

3 Ph.D., Montaserieh Research & Clinical Center for Infertility, Mashhad University of Medical Sciences, Mashhad, Iran

4 M.Sc. in Biology, Faculty of Sciences, Islamiz Azad University, Mashhad, Iran

Abstract

Introduction: Treatment of infertility by using in vitro matured oocytes derived from pre-ovulatory follicles has many potential applications. It minimizes the risk of ovarian hyperstimulation syndrome and is an alternative treatment for polycystic ovary syndrome.It also reduces costs and duration of treatment and can be important for subjects who need preservation. So provides information about final stage of oocyte maturation. We aimed to study the effects of recombinant follicle stimulating hormone (r-FSH) and human menopausal gonadotropin hormone (HMG) on human immature oocyte maturation in culture media and evaluated rate of eight-cell embryos mediated by intracytoplasmic sperm injection (ICSI).
Material and Methods: This experimental study was done in 2006-2007 in Montaserieh IVF Center and Mashhad Islamic Azad University. One hudred fifty six immature oocytes produced by women undergoing IVF were divided to three groups. In control group the oocytes were supplemented by 10% human serum albumin. In r-FSH group the oocytes were supplemented by 0.075 IU/ml r-FSH. And in the HMG group the oocytes were supplemented by 0.075 IU/ml HMG. Then matured oocytes were fertilized by ICSI and the numbers of embryos up to eight cells were investigated by invert microscope.
Results: The precentage of oocytes reaching metaphase 2 and the rate of eight-cell embryos was significantly higher in the FSH and HMG groups compared with the control group (p<0.001).
Conclusion: Using either HMG or r-FSH can increase the rate of human immature oocytes maturation in vitro. HMG significantly increased the production rate of eight-cell embryos compared with r-FSH (p<0.001).
 

Keywords

References

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