The Iranian Journal of Obstetrics, Gynecology and Infertility

The Iranian Journal of Obstetrics, Gynecology and Infertility

Effect of a period of swimming exercise and vitamin E intake on catalase and superoxide dismutase activity, and malondialdehyde levels in ovarian tissue of endometriosis model rats

Document Type : Original Article

Authors
Fatemeh Shahidian Akbar 1
Parvin Farzanegi 2
Hajar Abbaszadeh 3
1 PhD Candidate, Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
2 Associate Professor, Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
3 Assistant Professor, Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran.
10.22038/ijogi.2020.17374
Abstract
Introduction: Endometriosis is an estrogen-dependent inflammatory disease. Studies show that in women with endometriosis, the activity of macrophages in the peritoneal cavity increases, resulting in inflammatory reactions and stabilization of endometriosis. This study was performed with aim to investigate the effect of a period of swimming exercise and vitamin E intake on catalase and superoxide dismutase activity, and malondialdehyde levels in ovarian tissue of endometriosis model rats.
Methods: In this experimental study, 25 rats were randomly divided into 5 groups; healthy-control, endometriosis, endometriosis+exercise, endometriosis+vitaminE, endometriosis+vitaminE+exercise. To induce endometriosis, ovarian tissue was removed along with part of the fallopian tube tissue and the tissue pieces were grafted to the area of the right pelvic wall. Vitamin E intake was 200 mg / kg. Swimming training was eight weeks and 30 minutes each day. Data were analyzed by SPSS software (version 23) and one-way ANOVA and Tukey's post hoc tests. P < 0.05 was considered statistically significant.
Results: The groups of endometriosis+exercise, endometriosis+vitamin E and endometriosis+exercise+vitamin E showed a significant increase in Superoxide dismutase activity compared to the endometriosis group (P<0.0001) and endometriosis+exercise+vitamin E group compared to the endometriosis+exercise (P=0.0013) and endometriosis+vitamin E groups (P=0.0257). Also, a significant increase in Catalase activity was observed in endometriosis+exercise (P=0.0036), endometriosis+vitamin E (P=0.0006) and endometriosis+exercise+vitamin (P<0.0001) groups compared to the endometriosis group. However, the concentration of Malondialdehyde showed a significant decrease in endometriosis+exercise, endometriosis+vitamin E and endometriosis+exercise+vitamin E compared to the endometriosis group (P<0.0001).
Conclusion: The desired changes in the catalase and superoxide dismutase activity and malondialdehydelevels in the endometriosis process can improve the relative oxidative status of the disease in the animal model; performing the swimming exercises as well as the simultaneous use of vitamin E will help to improve oxidation indices in the animal model.
Keywords
Catalase
Endometriosis
Malondialdehyde
Superoxide dismutase
Swimming
Vitamin E
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The Iranian Journal of Obstetrics, Gynecology and Infertility
Volume 23, Issue 9
November 2021
Pages 43-51