The effect of eight weeks of aquatic aerobic training on lipid profile, Glucose, Insulin resistance and Apoprotein A and B in overweight postmenopausal women

Document Type : Original Article


1 M.Sc. in Sports Physiology, Faculty of physical education and sport sciences, Ferdowsi university of Mashhad, Mahhad, Iran.

2 Associated Professor of Department of Sports Physiology, Faculty of physical education and sport sciences, Ferdowsi university of Mashhad, Mahhad, Iran.

3 Assistant Professor of Department of Sport Pathology and Corrective Exercises, Faculty of physical education and sport sciences, Ferdowsi university of Mashhad, Mahhad, Iran.


Introduction: Menopausedue to low estrogen levels is associated with an increased fat percentage and particularly visceral fat and it is directly related to the risk of coronary heart diseases; So, the aim of this study was to investigate the effect of eight weeks of aquatic aerobic training on lipid profile, Glucose, Insulin resistance and Apoprotein A and B in overweight postmenopausal women.
Materials and Methods: This randomized clinical trial was performed on 26 postmenopausal women at Ferdowsi University of Mashhad in 2014. The subjects were randomly divided into two groups of Control and Exercise. The experimental group participated in Aquatic aerobic training for eight weeks. The three-point method and body composition device, as well as the Homa equation were used to determine the fat percentage, body mass index and insulin resistance, respectively. The related kits were also utilized for evaluation of cholesterol, low and high density lipoprotein, triglycerides, apolipoprotein A and B values. Analyze of data was done by SPSS software (version 16) and Kolmogorov-Smirnov test, dependent t-test and independent t-test. The value of p was considered significant as less than 0.05.
Results: Regular aquatic aerobic training caused a significant decrease in levels of apolipoprotein A (p=0.001) and apolipoprotein B (p=0.019), LDL (p=0.06), total cholesterol (p=0.03), glucose (p=0.038) and insulin resistance (p=0.003) and a significant increase in HDL (p=0.015); in this group, TG also decreased which was not significant (p=0.72). In Control group, levels of apolipoprotein A (p=0.029) and B (p=0.026), declined significantly; in this group, it was observed an significant increase in TG levels (p=0.027) and insignificant increase in total cholesterol (p=0.30) and glucose (p=0.80); insulin resistance decreased which was not significant (p=0.61).
Conclusion: The results indicate that eight weeks of aquatic aerobic training caused an improvement in some cardiovascular risk factors in overweight postmenopausal women and this kind of training is recommended to prevent the risk of atherosclerosis and cardiovascular diseases.


  1. Khazaei M, Moien-Afshari F, Laher I. Vascular‏ ‏endothelial function in health and ‎‎diseases. Pathophysiology 2008; 15(1):49-67. (Persian)‎‏.‏
  2. Lerner DJ, Kannel WB. Patterns of coronary heart disease morbidity and mortality in ‎the sexes: a 26-year follow-up of the Framingham population. Am Heart J ‎‎1986; 111(2):383-90‎.
  3. Mehdizadeh R. The effect of core stability training on body composition and lipoprotein ‎in menopausal older women. Iran J Age 2015; 10(2):156-63. (Persian)‎‏.
  4. Manley AF. Physical activity and health: a‏ ‏report of the surgeon general. ‎Collingdale, PA: DIANE Publishing; 1996‎‏.‏
  5. ‎Boroumand MA, Rekabi V, Davoodi G, Amirzadegan A, Saadat S, Abbasi SH, et al. Correlation between ‎lipoprotein(a) serum concentration and severity of coronary artery stenosis in an Iranian population according ‎to Gensini score. Clin Biochem 2008; 41(3):117-20‎.
  6. Ridker PM, Stampfer MJ, Rifai N. Novel risk factors for systemic atherosclerosis: a comparison of C-reactive protein, fibrinogen, homocysteine, lipoprotein(a), and standard cholesterol screening as predictors of peripheral arterial disease. JAMA 2001; 285(19):2481-5.
  7. Hosseinian M, Banitalebi E, Amirhosseini SE. Effect of 12 weeks of intensive interval and combined training on apolipoprotein A and B, visfatin and insulin resistance in overweight middle-aged women with type 2 diabetes. Horizon Med Sci 2016; 22(3):237-45.
  8. Boffa MB, Marcovina SM, Koschinsky ML. Lipoprotein(a) as a risk factor for atherosclerosis and ‎thrombosis: Mechanistic insights from animal models. Clin Biochem 2004; 37(5):333-43. ‎
  9. ‎Frohlich J, Al-Sarraf A. Cardiovascular risk and atherosclerosis prevention. Cardiovasc Pathol 2013; 22(1):16-8.‎
  10. ‎Superko HR. Advanced lipoprotein testing and sub fractionation are clinically useful. Circulation 2009; ‎‎119(17):2383-95.‎
  11. Greene NP, Martin SE, Crouse SF. Acute exercise and training alter blood lipid and lipoprotein profiles differently in overweight and obese men and women. Obesity 2012; 20(8):1618-27.
  12. Heilbronn LK, Noakes M, Morris AM, Kind KL, Clifton PM. 360His polymorphism of the apolipoprotein A-IV ‎gene and plasma lipid response to energy restricted diets in overweight subjects. Atherosclerosis 2000; 150(1):187-92.‎
  13. ‎Khuseyinova N, Koenig W. Apolipoprotein A-I and risk for cardiovascular diseases. Curr Atheroscler Rep ‎‎2006; 8(5):365-73.‎
  14. ‎Haidari M, Moghadam M, Chinicar M, Ahmadieh A, Doosti M. Apolipoprotein B as the best predictor of ‎coronary artery disease in Iranian normolipidemic patients. Clin Biochem 2001; 34(2):149-55.
  15. ‎Sarraf-Zadegan N, Sayed-Tabatabaei FA, Bashardoost N, Maleki A, Totonchi M, Habibi HR, et al. The ‎prevalence of coronary artery disease in an urban population in Isfahan, Iran. Acta Cardiol 1999; 54(5):257-‎‎63.
  16. ‎Strong JP, McGill HC Jr. The natural history of coronary atherosclerosis. Am J Pathol 1962; 40:37-49.‎
  17. ‎Atella V, Brady A, Catapano AL, Critchley J, Graham IM, Hobbs FD, et al. Bridging science and health policy in cardiovascular disease: Focus on lipid ‎management. A report from a session held during the 7th international symposium on multiple risk factors ‎in cardiovascular diseases: prevention and intervention--health policy, in Venice, Italy, on 25 October 2008. Atheroscler ‎Suppl 2009; 10(1):3-21.‎
  18. ‎Zhang L, Qin LQ, Liu AP, Wang PY. Prevalence of risk factors for cardiovascular disease and their ‎associations with diet and physical activity in suburban Beijing, China. J Epidemiol 2010; 20(3):237-43‎.
  19. Raffaelli C, Milanese C, Lanza M, Zamparo P. Water-based training enhances both physical capacities and body composition in healthy young adult women. Sport Sci Health 2016; 12(2):195-207.
  20. Sporis G, Ruzic L, Nedic A. The effects of aqua aerobic on patients with type II diabetes mellitus. Hrvat Sports Vijesnik 2013; 28(1):33-8.
  21. Kasprzak Z, Pilaczyńska-Szcześniak Ł. Effects of regular physical exercises in the water on the metabolic profile of women with abdominal obesity. J Hum Kinet 2015; 41(1):71-9.
  22. Haghighi AH, Yaghoubi M, HosseinI KS. The effect of eight weeks of aerobic training with green tea on serum ‎lipid profile and fat percent in obese ‎and overweight women.‎‏ ‏Med J Mashhad Univ Med Sci 2013; 56(4):211-8. (Persian).
  23. Gaeini A, Veysi M, Sheykh AV, Souri R. The effect of incremental aerobic training on A & B Apo protein in non-athlete men. Res Sport Sci 2011; 4(11):123-31. (Persian)‎‏.
  24. Boussuges A, Gavarry O. Exercise on land or in water? Int J General Med 2012; 5:313-6.
  25. Wiesner S, Birkenfeld AL, Engeli S, Haufe S, Brechtel L, Wein J, et al. Neurohumoral and metabolic response to exercise in water. Horm Metab Res 2010; 42(5):334-9.
  26. Kantyka J, Herman D, Roczniok R, Kuba L. Effects of aqua aerobics on body composition, body mass, lipid profile, and blood count in middle-aged sedentary women. Hum Movement 2015; 16(1):9-14.
  27. Alberti K, Zimmet PZ. Definition, diagnosis and classification of diabetes mellitus and its complications. ‎Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet Med 1998; 15(7):539-53.‎
  28. Leger LA, Lambert J, Martin P. Validity of plastic skinfold caliper measurements. ‎Hum Biol 1982; 5(3):667-75.‎‏
  29. de Castro JA, de Lima TR, Silva DA. Body composition estimation in ‎children and adolescents by bioelectrical impedance analysis: a systematic review. J ‎Bodywork Movement Ther 2017; 3(2):10-5.‎‏
  30. Hagner-Derengowska M, Kaluzny K, Kochanski B, Hagner W, Borkowska A, Czamara A, et al. Effects of Nordic Walking and Pilates exercise programs on blood glucose and lipid profile in overweight and obese postmenopausal women in an experimental, nonrandomized, open-label, prospective controlled trial. Menopause 2015; 22(11):1215-23.
  31. Rostamian M, Bijeh N. The effect of short-term aerobic exercise and green tea consumption on MFO,‎ Fatmax, body composition and lipid profile in sedentary postmenopausal‎‎ women. Int J Appl Exer Physiol 2017; 6(1):21-31.
  32. ‎Zoppini G, Targher G, Zamboni C, Venturi C, Cacciatori V, Moghetii P, et al. Effects of moderate-‎intensity exercise training on plasma biomarkers of inflammation and endothelial dysfunction in older patients ‎with type 2 diabetes. Nutr Metab Cardiovasc Dis 2006; 16(8):543-9.
  33. ‎Alam S, Stolinski M, Pentecost C, Boroujerdi MA, Jones RH, Sonksen PH, et al. The effect of a ‎six-month exercise program on very low-density lipoprotein apolipoprotein B secretion in type 2 diabetes. J Clin ‎Endocrinol Metab 2004; 89(2):688-94.
  34. Shahrjerdi M, Shavandi A, Golpayegani M, Sheykh Hosseini R. The effect of endurance and resistance trainings on blood glucose, quality of life amd mental health in women with diabete 2. J Diabetes Metab 2009; 9(1):35-44. (Persian).
  35. Koozehchian MS, Nazem F, Kreider RB, Roberts WJ, Best TM, Rong Y, et al. The role of exercise training on lipoprotein profiles in adolescent males. Lipids Health Dis 2014; 13:95.
  36. Ketabipoor SM, Koushkie JM. Effeet of aquatic aerobic training on serum A and B apoproteins and lipoproteins in obese and normal weight menopause women. Arak Med Univ J 2014; 17(8):44-52. (Persian)‎‏.
  37. Safari Farsani F, Azamian Jazi A. Effects of aerobic exercise on lipid profile and apoprotein A and B in overweight women. The Sixth National Conference on Physical Education and Sport Sciences Students, Institute of Physical Education and Sport Sciences, Tehran,‏ Iran; 2011. (Persian)‎‏.