Comparison of Pregnancy Rate and Effective Factors following Fresh and Frozen Embryo Transfer in Women undergoing Assisted Reproductive Techniques (ART)

Document Type : Original Article

Authors

1 Resident, Department of Obstetrics and Gynecology, Women's Health Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

2 Professor, Department of Obstetrics and Gynecology, Women's Health Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Professor, Department of Biostatistics, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract

Introduction: Embryo cryopreservation is potentially the best method to increase pregnancy rate for the women at risk of OHSS (Ovarian Hyper stimulation Syndrome) and the women at risk of losing ovarian function. With regard to the importance of issue and increasing development in the world regarding to Embryo cryopreservation and following pregnancy, this study was performed with aim to compare pregnancy rate and effective factors following fresh and frozen embryos transfer in women undergoing Assisted Reproductive Techniques (ART) in Milad Infertility Center (Montaserieh).
Methods: This cohort study was performed on 200 infertile women who had referred to Mashhad Montaserieh Infertility Center during 2009-2010 for infertility treatment and were candidate for IVF/ICSI. Mature embryos of the patients were used in frozen and fresh groups for embryo transfer to uterus. Data was analyzed by SPSS software (version 11.5) and using Chi-square, independent t, and Kruskal Wallis test. P<0.05 was considered significant.
Results: From 200 infertile women, 80 cases (42%) had pregnancy which 43 (43%) were in fresh embryo group and 37 (37%) in frozen embryo group; the difference was not significant (P=0.38). Infertility causes, number of embryo cells and grading of transferred embryos, delivery complications, method of embryo transfer, number of fetuses obtained from delivery, and endometrial thickness were not statistically different between two groups (P=0.61). But, mean number of transferred embryo was statistically different between fresh embryo group and frozen embryo group (P<0.05), but this difference was not significant when non pregnant women were deleted and comparison among pregnant women was not significant (P=0.22).
Conclusion: There was no significant difference between fresh and frozen groups in terms of pregnancy rate and pregnancy complications (P=0.53).

Keywords

References

  1. Cobo A, Meseguer M, Remohi J, Pellicer A. Use of cryo-banked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial. Hum Reprod 2010; 25(9):2239–46.
  2. Maroufizadeh S, Ghaheri A, Omani Samani R. Factors associated with poor quality of life among Iranian infertile women undergoing IVF. Psychol Health Med 2016; 22(2):145-51.
  3. Schoolcraft WB, Surrey ES, Gardner DK. Embryo transfer: techniques and variables affecting success. Fertil Steril 2001; 76(5):863–70.
  4. Nouri K, Tempfer CB, Walch K, Promberger R, Dag S, Ott J. Predictive value of the time interval between embryo loading and transfer for IVF/ICSI success: a prospective cohort study. Reprod Biol Endocrinol 2015; 13:51.
  5. Doody KJ. Cryopreservation and delayed embryo transfer–assisted reproductive technology registry and reporting implications. Fertil Steril 2014; 102(1):27–31.
  6. Shapiro BS, Daneshmand ST, Restrepo H, Garner FC, Aguirre M, Hudson C. Matched-cohort comparison of single-embryo transfers in fresh and frozenthawed embryo transfer cycles. Fertil Steril 2013; 99(2):389–92.
  7. Halvaei I, Khalili MA, Razi MH, Agha-Rahimi A, Nottola SA. Impact of different embryo loading techniques on pregnancy rates in in vitro fertlization/embryo transfer cycles. J Hum Reprod Sci 2013; 6(1):65–9.
  8. Mains L, Van Voorhis BJ. Optimizing the technique of embryo transfer. Fertil Steril 2010; 94(3):785–90.
  9. Cobo A, Castello D, Vallejo B, Albert C, de los Santos JM, Remohi J. Outcome of cryotransfer of embryos developed from vitrified oocytes: double vitrification has no impact on delivery rates. Fertil Steril 2013; 99(6):1623–30.
  10. Xue Y, Tong X, Jiang L, Zhu H, Yang L, Zhang S. Effect of vitrification versus slow freezing of human day 3 embryos on β-hCG levels. J Assist Reprod Genet 2014; 31(8):1037–43.
  11. Scaravelli G, Vigiliano V, Mayorga JM, Bolli S, De Luca R, D'Aloja P. Analysis of oocyte cryopreservation in assisted reproduction: the Italian National Register data from 2005 to 2007. Reprod Biomed Online 2010; 21(4):496-500.
  12. Noreh LJ, Tucs O, Sekadde-Kigondu CB, Noreh JA. Outcomes of assisted reproductive technologies at the Nairobi In Vitro Fertilisation Centre. East Afr Med J 2009; 86(4):156-61.
  13. Hunault CC, Eijkemans MJ, Pieters MH, te Velde ER, Habbema JD, Fauser BC, et al. A prediction model for selecting patients undergoing in vitro fertilizaiton for elective single embryo transfer. Fertil Steril 2002; 77(4):725–32.
  14. Kuwayama M, Vajta G, Kato O, Leibo SP. Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online 2005; 11(3):300–8.
  15. Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Hudson C, Thomas S. Evidence of impaired endometrial receptivity after ovarian stimulation for in vitro fertilization: a prospective randomized trial comparing fresh and frozen-thawed embryo transfer in normal responders. Fertil Steril 2011; 96(2):344–8.
  16. Vahid RF, Ayati S, Mirzaeean S, Shakeri MT. Fertility outcome after IVF and related factors. J Gorgan Univ Med Sci 2009; 11(3):42-6. (Persian).
  17. Roque M, Lattes K, Serra S, Solà I, Geber S, Carreras R, et al. Fresh embryo transfer versus frozen embryo transfer in in vitro fertilization cycles: a systematic review and meta-analysis. Fertil Steril 2013; 99(1):156-62.
  18. Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Hudson C, Thomas S. Similar ongoing pregnancy rates after blastocyst transfer in fresh donor cycles and autologous cycles using cryopreserved bipronuclear oocytes suggest similar viability of transferred blastocysts. Fertil Steril 2010; 93(1):319–21.
  19. Griesinger G, Schultz L, Bauer T, Broessner A, Frambach T, Kissler S. Ovarian hyperstimulation syndrome prevention by gonadotropin-releasing hormone agonist triggering of final oocyte maturation in a gonadotropin-releasing hormone antagonist protocol in combination with “freeze-all” strategy: a prospective multicentric study. Fertil Steril 2011; 95(6):2029–33.
  20. D'Angelo A. Ovarian hyperstimulation syndrome prevention strategies: cryopreservation of all embryos. Semin Reprod Med 2010; 28(6):513–8.
  21. Salim R, Ben-Shlomo I, Colodner R, Keness Y, Shalev E. Bacterial colonization of the uterine cervix and success rate in assisted reproduction: results of a prospective survey. Hum Reprod 2002; 17(2):337-40.
The Iranian Journal of Obstetrics, Gynecology and Infertility
Volume 20, Issue 6
August 2017
Pages 24-30

Files

Share

How to cite

Statistics