The Iranian Journal of Obstetrics, Gynecology and Infertility

The Iranian Journal of Obstetrics, Gynecology and Infertility

Association between polymorphism of has-miR-125 (rs12976445) and susceptibility to idiopathic recurrent pregnancy loss in Iranian women

Document Type : Original Article

Authors
Sara Nemati Vahedi 1
Babak Kheirkhah 2
Ali Akbar Malekirad 3
1 PhD student in Molecular Cell Biology, Faculty of Basic Sciences, Islamic Azad University Sirjan Branch, Sirjan, Iran.
2 Assistant Professor, Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University Baft Branch, Baft, Iran.
3 Associate Professor, Department of Biology, Faculty of Basic Sciences, Payame Noor University, Tehran, Iran.
10.22038/ijogi.2020.17292
Abstract
Introduction: Many new studies related to peri-implantation period and maintenance of pregnancy suggest that microRNAs have fundamentally role in regulating critical pathways in early pregnancy loss and implantation failure. This study was performed with aim to evaluate the possible association between has-miR-125 T>C polymorphism and susceptibility to idiopathic recurrent pregnancy loss (RPL).
Methods: In this case-control study, 116 females with at least two or more recurrent pregnancy loss and 89 healthy women with a history of two successful pregnancies and without a history of miscarriage were evaluated. DNA molecule was extracted by RGDE method and genotyping was performed by ARMS Tetra method. Data were analyzed by SPSS software (version 22) and Chi-squared test. P<0.05 was considered statistically significant.
Results: The frequency of TT, TC and CC genotypes of rs12976445 polymorphism were 50.86%, 42.25% and 6.89% and in women with RPL and were 58.43%, 39.33% and 2.24% in healthy women. There was no significant relationship between rs12976445 polymorphism and recurrent pregnancy loss (P=0.238).
Conclusion: No significant association was found between rs12976445 polymorphism and RPL.
Keywords
miR-125
Recurrent pregnancy loss (RPL)
Single nucleotide polymorphism (SNP)
Tetra -ARMS PCR
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The Iranian Journal of Obstetrics, Gynecology and Infertility
Volume 23, Issue 8
October 2020
Pages 37-48