Investigation of codons 164 and 27 of beta 2-adrenergic receptor gene polymorphism in women with the polycystic ovary syndrome

Document Type : Original Article


1 Associate Professor, Vali-e-Asr Reproductive Health Research Center, Vali-e-Asr Hospital, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran

2 Instructor, Department of Community Medicine, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran

3 M.Sc. student, Department of Biology, Young Researchers Club, School of Pharmaceutical Sciences, Tehran Islamic Azad University, Tehran, Iran

4 Ph.D. student of Genetics, Department of Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


Introduction: Polycystic ovary syndrome (PCOS) is a common and complex disease in women. Although the role of genetic factors in PCOS is strongly supported, the genes that are involved in the etiology of this syndrome have not yet been fully investigated. The aim of this study was the investigation of codons 164 and 27 of beta 2-adrenergic receptor gene polymorphism in women with PCOS.
Methods: This cross-sectional study was performed in 2014 on 100 women with PCOS in Imam Khomeini Hospital, Tehran, Iran. 3 ml blood samples were collected for extraction of genomic DNA by the Qiagen DNA extraction kit,  then gene bank based primers were prepared for PCR. Approved DNA samples were sequenced by Macrogen (row or sequence); resulting sequences were evaluated by the Chromas (ver. 2.4) application. The data were analyzed using the bootstrap method and SPSS software (ver.18). P < 0.05 was considered significant.
Findings: The analysis of polymorphism in codon 164 in PCO women showed 44.4% statistically significant polymorphism (P<0.002). Codon 27 did not show any significant polymorphism differences between PCO and non-PCO groups (P=0.606). Homozygous and heterozygous in both codons were significantly different (P=0.004 and P=0.0450, respectively).   
Conclusion: Inspection of beta2 adrenoceptor gene polymorphism in codons 164 and 27 showed that codon 164 is associated with PCO.


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