Molecular Analysis and Comparison of Y chromosome Microdeletions in Tabriz and Kashan Infertile Men with Azoospermia and Severe Oligospermia

Torfeh, Mahnaz; Sakhinia, Ebrahim; Hasani, Hasan; Ahmadi Asre Badr, Yadollah; Nourizadeh, Davoud; Heshmat, Yaghoub; Rohani, Mahdi

doi:10.22038/ijogi.2012.5740

Molecular Analysis and Comparison of Y chromosome Microdeletions in Tabriz and Kashan Infertile Men with Azoospermia and Severe Oligospermia

Document Type : Original Article

Authors

Mahnaz Torfeh ¹

Ebrahim Sakhinia ²

Hasan Hasani ³

Yadollah Ahmadi Asre Badr ⁴

Davoud Nourizadeh ⁴

Yaghoub Heshmat ⁵

Mahdi Rohani ⁶

¹ M.Sc. of Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

² Assistant Professor of Genetics, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

³ Associate Professor of Anatomy, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran.

⁴ Assistant Professor of Urology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

⁵ Assistant Professor of Genetics, Genetic Diagnosis Center, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.

⁶ Ph.D Student of Bacteriology, Department of Microbiology, Pastor Institute, Tehran, Iran.

10.22038/ijogi.2012.5740

Abstract

Introduction: Studies has shown that approximately 10-15% of married couples have infertility problem and a male factor can be accounted for half of these cases. Deletion in three non- overlapping regions on long arm of Y chromosome (AZFa, AZFb and AZFc) causes defective spermatogenesis and ultimately infertility in men. According to recent studies males who have Y chromosome deletions and they are by ICSI for infertility may transmit their deletions to a son offspring. So before ICSI all such men should undergo screening for the presence of these deletions in order to prevent the transmission to their male offspring.
Methods: Screening of Y chromosome micro deletions was done in 100 infertile men who came to infertility center of Beheshti hospital in Kashan and infertility centers in Tabriz for ICSI. Genomic DNA was extracted from peripheral blood lymphocytes by standard ethanol chloroform techniques. By using 7 STS markers based on EAA/EMQN guideline and 11 STS markers which used in Iran and neighbor countries, PCR performed on each samples for detection of micro deletions.
Results: In Kashani participants, none of the patients had these deletions, but in Tabrizi patients 4 (8%) had these deletions.
Conclusion: This study failed to find greatly deletion in the patients who referred for ICSI in our study, so it appears that other factors were important in the infertility in this population.

Keywords

Infertility

Micro deletions

Y chromosome

Molecular analysis

Azoospermia

Oligospermia

Infertile men