Investigating the Effects of Hydro-Alcoholic Extract of Leaves of Henna on Mouse Embryo Balb/C

Document Type : Original Article

Authors

1 Assistant Professor of Obstetrics and Gynecology, Medicinal Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

2 M.Sc. of Histology, Medicinal Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

3 B.Sc. of Midwifery, Medicinal Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

4 General Practitioner, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

5 Assistant Professor of Medicinal Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

6 M.Sc. student of animal physiology, Payam Noor University of Isfahan, Isfahan, Iran.

7 Associate Professor of Biostatistics, Plant Biochemistry Clinical Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

8 Doctor of Veterinary Medicine, Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

9 Professor of Pharmacology, Medicinal Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Abstract

Introduction: According to the traditional believes Henna causes abortion; however, there is no scientific study in this regard and about its teratogenic effect. The aim of this research was to study the effect of Henna extract on congenital malformations in mouse.
Methods: In this experimental study, a total of 120 female mice (30-40 gr, 8-12 week old) after mating and observing vaginal plug, were randomly allocated in 4 groups of control (Without intervention and distilled water injection) and case (Concentrations of 10 and 100 milligram per kilogram of Henna extract). All groups received daily intra-peritoneal injection for seven days. Cesarean operation was held on 18th and 19th day, as well. While height and weight measurements, embryos were examined for abnormalities. Data were analyzed by SPSS software version 11.5, ANOVA and least significant difference (LSD) test. P value less than 0.05 was statistically significant.
Results: A significant difference was observed in weight and length of fetuses in case and control group (p<0.001) but there is no relevant difference among groups that received Henna. 
In 90% of case group, fetuses had no parietal bone and more extra ribs were observed in the groups who receiving Henna extract.
Conclusion: Hydro-alcoholic extract of Henna has teratogenic properties, so it should be consumed with caution during pregnancy.

Keywords

References

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