Document Type : Original Article
Authors
1
Tehran
2
1- Noorgene Genetic & Clinical Laboratory, Molecular Research Center, Ahvaz, IRAN.
3
2- High Institute for Education and Research in Transfusion Medicine, Tehran, Iran.
4
3- Shahid AkbarAbadi Clinical Research Development unit (SHACRDU), School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
5
4- Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACER, Tehran, Iran.
10.22038/ijogi.2025.89527.6485
Abstract
Background: High blood pressure in preeclampsia is one of the most important complications in these patients, which increases the risk of cardiovascular disease. Given that genes play an important role in the occurrence of blood pressure through biological processes, the use of bioinformatics analysis, which is a new and innovative method, can be effective in identifying influential genes.
Method: The GEO database was used to extract data. The extracted databases included GSE48424, GSE99007, and GSE91189. These data were related to patients with severe and non-severe forms of preeclampsia. The criteria selected for the data included |logFC| greater than one and p<0.05. Finally, data analysis was performed using the GEO2R tool.
Result: After clustering genes for the dataset, 235 highly expressed genes and 546 low expressed genes were obtained in non-severe and severe forms of preeclampsia. The results showed that the changes in gene expression between patients and healthy individuals were mainly involved in biological processes including inflammation, cell adhesion, and cell metabolism. The genes that had the most important roles and the most correlations with other genes were HLTF, SUMO1, KDR, SNRPD3, DERL2, VCP, EIF4B, NOTCH1, SOCS3, CBL, ICAM-1, ITGB2, STRN, MEF2A, and PTPRC. Also, the most important microRNAs were miR-3135-3P, miR-5085, and miR-6-5085.
Conclusion: It can be said that identifying the upstream and downstream pathways of genes can probably help in designing preventive and therapeutic strategies, which requires further studies.
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