DNA methylation of CDKN2A and CDKN2B genes in pregnant women infected and non-infected with gestational diabetes

Document Type : Original Article

Authors

1 M.Sc. in Anatomical Sciences, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

2 Associate Professor, Department of Genetics, Medical Cellular and Molecular Research Center, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

3 Professor, Department of Anatomical Sciences, Gorgan Congenital Malformations Research Center, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran.

Abstract

Introduction: Diabetes is one of the metabolic diseases characterized by hyperglycemia and disruption of glucose homeostasis. Gene methylation is one of the epigenetic mechanisms that results in the silencing of the desired gene. CDKN2A and CDKN2B genes are associated with gestational diabetes. The present study was conducted with aim to compare the DNA methylation level of CDKN2A and CDKN2B genes in women infected and non-infected with gestational diabetes.
Methods: This case-control study was carried out during 2020 on 48 pregnant women in age range of 18-35 years in 24-28 weeks of gestation including 24 pregnant women with gestational diabetes (case group) and 24 pregnant women without gestational diabetes (control group) that had referred to Shahid Sayad Shirazi Medical Education Center, Gorgan. After DNA extraction from blood, the methylation of the genes was investigated by bisulfite sequencing, and then the desired gene fragment was sequenced, and the number of methylated and unmethylated CPGs was analyzed. The data were analyzed by SPSS software (version 16) and student t-test. P<0.05 was considered significant.
Results: The mean percentage of CDKN2A gene methylation in the case group (19.79±2.07) was lower than the control group (33.54±2.54) (P<0.05). Also, the mean percentage of CDKN2B gene methylation in the case group (29.91±2.85) was lower than the control group (45.45±2.98) (P<0.05).
Conclusion: The findings of the present study indicated lower values of the mutilation of CDKN2A and CDKN2B genes in pregnant woman with gestational diabetes compared to the pregnant women without gestational diabetes. 

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References

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The Iranian Journal of Obstetrics, Gynecology and Infertility
Volume 27, Issue 5
July 2024
Pages 32-40

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