Document Type : Original Article
Authors
1
physiology sport.sari.iran
2
sariAssociated Professor, Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, I. R. Iran.
3
Assistant Professor, Department of Exercise Physiology, Sari Branch, Islamic Azad University, Sari, I. R. Iran.
4
Assistant Professor, Department of Exercise Physiology, Ghaemshahr Branch, Islamic Azad University, Ghaemshahr, I. R. Iran
10.22038/ijogi.2024.77433.5972
Abstract
Background and purpose: Infertility is actually the lack of fertility in a couple who have not conceived in spite of trying to reproduce for one years. The purpose of this research is to investigate the effect of swimming exercise, vitamin E and stem cell injection on testosterone and Lc31 and P62 genes in the testicular tissue of azoospermia model mice.
Materials and methods: In this experimental study, 40 6- to 8-week-old rats were randomly selected from Tehran laboratory animal breeding and research center, and then the azoospermia model was induced with busulfan at a dose of 40 mg. After one month, the rats were divided into 8 groups; control, patient, sham, patient + exercise, patient + supplement, patient + cell, patient + supplement + exercise and patient + cell + exercise (each group has 5 heads). The group of cells and cells + one-time training stem cells were transplanted in the area of the vas deferens in the amount of one million cells for each mouse. The supplement and training group received vitamin E oral solution supplement at the rate of 100 mg/kg by gavage. Also, training groups swam 30 minutes a day, 5 days a week for 8 weeks.
Results: Induction of azoospermia significantly decreased testosterone levels and the expression of Lc31 and P62 genes in testicular tissue compared to the control group (P≤0.05), but in the supplement, cell, exercise, exercise+supplement and exercise+cell groups compared to the patient and sham groups showed a significant increase (P≤0.05).
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