Document Type : Original Article
Authors
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1. Zanjan Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran 2. Biochemistry department, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran 3. Faculty of Biological Sciences, Tarbiat Modares
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Department of Obstetrics and Gynecology, Mousavi Hospital, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
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Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
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Biochemistry department, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
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Department of Biology, Faculty of Basic Sciences, University of Maragheh, Maragheh, Iran.
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Department of Pediatrics, Mousavi Hospital, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
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Zanjan Metabolic Diseases Research Center, Zanjan University of Medical Sciences, Zanjan, Iran
10.22038/ijogi.2024.75355.5877
Abstract
This study aimed to investigate whether the expression of CYFIP1 (as a key factor in ASD pathogenesis) in the umbilical cord blood (UCB) changed based on the maternal pre-pregnancy BMI and its potential association with different categories of maternal BMI, lipid profile, birth-weight, and status of newborn concerning gestational age.
Materials and methods: UCB samples from 118 newborns were categorized into three groups based on pre-pregnancy maternal BMI: normal weight (18.5≤BMI<25kg/m2, n=39), overweight (25≤BMI<30, n=41), and obese (BMI≥30kg/m2, n=38). The lipid profile including low-density lipoprotein (LDL), triglyceride (TG), total cholesterol (TC), and high-density lipoprotein (HDL) was measured in UCB. In addition, peripheral blood mononuclear cell purification and mRNA extraction were performed on the remaining sample for analysis of gene expression of CYFIP1 using real-time PCR.
Results: The expression of the CYFIP1 gene was elevated in UCB from women classified as overweight or obese compared to those with normal weight. UCB of obese women exhibited higher cholesterol and LDL levels compared to normal-weight and overweight women. Positive correlations were observed between maternal BMI and cord blood CYFIP1 gene expression (r=0.333), as well as cholesterol (r=0.520), TG (r=0.290), LDL (r=0.397), and status of gestational age (r=0.262).
Conclusion: Increased expression of the CYFIP1 gene is correlated positively with different categories of pre-pregnancy BMI and lipid profile, implying that risk factors contributing to increasing BMI may have negative consequences on fetal health and development. Exploring UCB genes associated with fetal neural development, at birth, can offer valuable insights for its early detection and intervention.
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