Epstein-Barr virus (EBV) frequency in tissue samples of women with breast cancer in Mashhad, Iran

Document Type : Original Article

Authors

1 Resident, Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

2 Assistant professor of Blood and Cancer of Adult, Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

3 Medical Student, Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

4 Assistant Professor of Molecular Medicine, Cancer Molecular Pathology Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

5 Assistant professor, Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Abstract

Introduction: Breast cancer is one of the most common cancers in the world that its incidence rate has increased over the last decade. Although the causes and risk factors of breast cancer are not fully known, studies have been reported on the relationship between breast cancer and viral agents, including the Epstein-Barr virus (EBV). Therefore, the present study was performed with aim to explore the correlation between EBV and breast cancer.
Methods: In this cross-sectional study which was conducted using breast tissue pathology samples collected from Mashhad hospitals between 2017 and 2022, 30 paraffin tissue samples from breast cancer patients and 33 paraffin tissue samples as a control group from benign breast diseases were collected. After the DNA extraction processes, the EBV virus was tested by polymerase chain reaction (PCR). Data analysis was done using SPSS software (version 20) and Chi-square, Fisher's exact and t-test. P<0.05 was considered statistically significant.
Results: According to the PCR results of 63 tissue biopsy samples, none of the malignant and control group samples were EBV-positive.
Conclusion: The presence of the EBV virus gene was not confirmed in a group of women with breast cancer in Mashhad. However, further studies with a larger sample size are needed to validate the results.

Keywords

Main Subjects

References

  1. Harbeck N, Gnant M. Interpretation of the evidence for the efficacy and safety of statin therapy. Lancet 2017; 389(10074):1134-50.
  2. da Costa Vieira RA, Biller G, Uemura G, Ruiz CA, Curado MP. Breast cancer screening in developing countries. Clinics 2017; 72:244-53.
  3. Scagliotti MF, Boietti BR, Knoblovits P. Prevalence of men's health history in male breast cancer patients. Endocrinología, Diabetes y Nutrición (English ed.) 2023; 70(6):415-20.
  4. Ghoncheh M, Pournamdar Z, Salehiniya H. Incidence and mortality and epidemiology of breast cancer in the world. Asian Pacific journal of cancer prevention 2016; 17(S3):43-6.
  5. Harirchi I, Karbakhsh M, Kashefi A, Momtahen AJ. Breast cancer in Iran: results of a multi-center study. Asian pacific journal of cancer prevention 2004; 5(1):24-7.
  6. Sun YS, Zhao Z, Yang ZN, Xu F, Lu HJ, Zhu ZY, et al. Risk factors and preventions of breast cancer. International journal of biological sciences 2017; 13(11):1387-97.
  7. Siegel RL, Miller KD, Fuchs HE, Jemal A. Cancer statistics, 2022. CA: a cancer journal for clinicians 2022; 72(1):7-33.
  8. Apovian CM. Obesity: definition, comorbidities, causes, and burden. Am J Manag Care 2016; 22(7 Suppl):s176-85.
  9. Ahlgren M, Melbye M, Wohlfahrt J, Sørensen TI. Growth patterns and the risk of breast cancer in women. International Journal of Gynecological Cancer 2006; 16:569-75.
  10. McCormack VA, dos Santos Silva I. Breast density and parenchymal patterns as markers of breast cancer risk: a meta-analysis. Cancer Epidemiology Biomarkers & Prevention 2006; 15(6):1159-69.
  11. Zhang Y, Kiel DP, Kreger BE, Cupples LA, Ellison RC, Dorgan JF, et al. Bone mass and the risk of breast cancer among postmenopausal women. New England Journal of Medicine 1997; 336(9):611-7.
  12. Hormones E, Breast Cancer Collaborative Group. Steroid hormone measurements from different types of assays in relation to body mass index and breast cancer risk in postmenopausal women: reanalysis of eighteen prospective studies. Steroids 2015; 99:49-55.
  13. Brettes JP, Mathelin C. Dual effects of androgens on mammary gland. Bulletin du cancer 2008; 95(5):495-502.
  14. Colditz GA, Rosner B. Cumulative risk of breast cancer to age 70 years according to risk factor status: data from the Nurses' Health Study. American journal of epidemiology 2000; 152(10):950-64.
  15. Collaborative Group on Hormonal Factors in Breast Cancer. Familial breast cancer: collaborative reanalysis of individual data from 52 epidemiological studies including 58 209 women with breast cancer and 101 986 women without the disease. The Lancet 2001; 358(9291):1389-99.
  16. Alsop K, Fereday S, Meldrum C, DeFazio A, Emmanuel C, George J, et al. BRCA mutation frequency and patterns of treatment response in BRCA mutation–positive women with ovarian cancer: a report from the Australian Ovarian Cancer Study Group. Journal of Clinical Oncology 2012; 30(21):2654-63.
  17. Gram IT, Park SY, Kolonel LN, Maskarinec G, Wilkens LR, Henderson BE, et al. Smoking and risk of breast cancer in a racially/ethnically diverse population of mainly women who do not drink alcohol: the MEC study. American journal of epidemiology 2015; 182(11):917-25.
  18. Jin QE, Su J, Yan D, Wu S. Epstein-Barr virus infection and increased sporadic breast carcinoma risk: a meta-analysis. Medical Principles and Practice 2020; 29(2):195-200.
  19. De Villiers EM, Sandstrom RE, Zur Hausen H, Buck CE. Presence of papillomavirus sequences in condylomatous lesions of the mamillae and in invasive carcinoma of the breast. Breast cancer research 2004; 7:1-11.
  20. Kaveh F, Amini K, Sadeh M. Prevalence of Herpes Simplex Virus type 1 and 2 (HSV-1 and HSV-2) in the women with breast cancer by Multiplex-PCR method. The Iranian Journal of Obstetrics, Gynecology and Infertility 2018; 21(3):39-44.
  21. Hosseinpouri P, Hejazi SH, Hadi F. Relationship between human papillomavirus and breast cancer in women with this cancer in Khuzestan province. The Iranian Journal of Obstetrics, Gynecology and Infertility 2020; 23(8):66-74.
  22. Salahshor Z, Hejazi H, Hadi F, Saeedi Z. the study of relationship between epstein-barr virus and breast cancer in isfahan province. Iranian Journal of Breast Diseases. 2018 Sep 10;11(2):16-24.
  23. Kamińska M, Ciszewski T, Łopacka-Szatan K, Miotła P, Starosławska E. Breast cancer risk factors. Menopause Review/Przegląd Menopauzalny 2015; 14(3):196-202.
  24. Delgado-García S, Martínez-Escoriza JC, Alba A, Martín-Bayón TA, Ballester-Galiana H, Peiró G, et al. Presence of human papillomavirus DNA in breast cancer: a Spanish case-control study. BMC cancer 2017; 17:1-11.
  25. Keyvani H, Fazlalipour M, Monavari SH, Mollaie HR. Hepatitis C virus-proteins, diagnosis, treatment and new approaches for vaccine development. Asian Pacific Journal of Cancer Prevention 2012; 13(12):5917-35.
  26. Salehi-Vaziri M, Sadeghi F, Alamsi-Hashiani A, Haeri H, Monavari SH, Keyvani H. Merkel cell polyomavirus and human papillomavirus infections in cervical disease in Iranian women. Archives of virology 2015; 160:1181-7.
  27. Farrell PJ. Epstein–Barr virus and cancer. Annual Review of Pathology: Mechanisms of Disease 2019; 14:29-53.
  28. Hoover K, Higginbotham K. Epstein-Barr Virus. [Updated 2023 Aug 8]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK559285/
  29. Hu H, Luo ML, Desmedt C, Nabavi S, Yadegarynia S, Hong A, et al. Epstein–Barr virus infection of mammary epithelial cells promotes malignant transformation. EBioMedicine 2016; 9:148-60.
  30. Fina F, Romain S, Ouafik LH, Palmari J, Ayed FB, Benharkat S, et al. Frequency and genome load of Epstein-Barr virus in 509 breast cancers from different geographical areas. British journal of cancer 2001; 84(6):783-90.
  31. Kalkan A, Ozdarendeli A, Bulut Y, Yekeler H, Cobanoglu B, Doymaz MZ. Investigation of Epstein-Barr virus DNA in formalin-fixed and paraffin-embedded breast cancer tissues. Medical Principles and Practice 2005; 14(4):268-71.
  32. Dowran R, Joharinia N, Safaei A, Bakhtiyarizadeh S, Alidadi Soleimani A, Alizadeh R, et al. No detection of EBV, BKV and JCV in breast cancer tissue samples in Iran. BMC research notes. 2019 Dec; 12(1):1-5.
  33. Kadivar M, Monabati A, Joulaee A, Hosseini N. Epstein-Barr virus and breast cancer: lack of evidence for an association in Iranian women. Pathology & Oncology Research 2011; 17:489-92.
  34. Huo Q, Zhang N, Yang Q. Epstein-Barr virus infection and sporadic breast cancer risk: a meta-analysis. PloS one 2012; 7(2):e31656.
  35. Amarante MK, Watanabe MA. The possible involvement of virus in breast cancer. Journal of cancer research and clinical oncology 2009; 135:329-37.
The Iranian Journal of Obstetrics, Gynecology and Infertility
Volume 26, Issue 12
February 2024
Pages 30-38

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