The effect of 8 weeks of swimming training and stem cell injection on some effective factors in the angiogenesis pathway of premature ovarian failure model rats

Document Type : Original Article


1 PhD student in Exercise Physiology, Department of Exercise Physiology, School of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.

2 Associate Professor, Department of Exercise Physiology, School of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.

3 Assistant Professor, Department of Exercise Physiology, School of Humanities, Sari Branch, Islamic Azad University, Sari, Iran.

4 Assistant Professor, Department of Biological Sciences and Technologies, School of Medical Sciences, Sari Branch, Islamic Azad University, Sari, Iran.



Introduction: Female infertility is a universal medical condition that can be caused by various disorders of the reproductive system, including premature ovarian failure. The present study was performed with aim to investigate the effect of 8 weeks of swimming training and stem cell injection on VEGF, TGF and HGF genes in the ovarian tissue of premature ovarian failure model rats.
Methods: In this experimental research, 30 female rats 6- to 8-week-old were selected. In order to create a model of premature ovarian failure, cyclophosphamide and biosulfan were used in the amount of 100 and 50 mg/kg, respectively, as an intraperitoneal injection. After model induction, rats were randomly divided into 6 groups (5 heads in each group) including: 1) healthy control, 2) patient+ sham, 3) patient+ saline, 4) patient+ cell, 5) patient +exercise and 6) patient+ cell+ exercise. The rats of the training group swam for 8 weeks. Cell groups, 2 weeks after creating the model stem cells were transplanted in the ovary at the rate of one million cells for each mouse. Data analysis was done using SPSS statistical software (version 22) and one-way ANOVA and Tukey's post hoc tests. P≤0.05 was considered significant.
Results: Induction of premature ovarian failure led to a significant increase in VEGF, TGF and HGF genes in the ovarian tissue of rats compared to the control-healthy group (P≤0.05) that the exercise, cell and exercise+ cell groups decreased the expression of these genes compared to the patient and patient-saline groups (P≤0.05).
Conclusion: The synergistic effect of aerobic exercise and bone marrow-derived mesenchymal stem cells improve angiogenic factors in experimental premature ovarian failure model rats.


Main Subjects

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