Tracking of bovine leukemia virus in blood and breast paraffin tissue samples

Document Type : Original Article


1 M.Sc. of Molecular and Cellular Biology, Department of Microbiology, School of Sciences, Islamic Azad University, Kerman, Iran.

2 Assistant Professor, Department of Microbiology, School of Basic Sciences, Kerman Branch, Islamic Azad University, Kerman, Iran.


Introduction: Breast cancer is one of the most important cancers in women. Research has been done in many parts of the world to investigate the presence of bovine leukemia virus (BLV) and its correlation with breast cancer on breast paraffin tissue and human blood samples. The present study was performed with aim to trace this virus in paraffin tissue and blood samples and to investigate the relationship between this virus and breast cancer in the studied population.
Methods: In this case-control study conducted in 2022, the presence of Gag gen of BLV was investigated in 220 samples (100 breast paraffin tissue samples and 120 DNA samples extracted from the blood of cancerous and healthy individuals) by using Nested-PCR method. Data were analyzed by SPSS software (version 26) and chi-square test. P<0.05 was considered statistically significant.
Results: Among 120 blood samples (13%) and among 100 breast paraffin tissue (8%) showed BLV infection that indicate better detection of this virus in the blood, but in comparison between patient and healthy samples, just paraffin tissue samples showed a significant relationship with breast cancer(p<0.05).
Conclusion:  Although there was a significant relationship between BLV infection and cancer in breast paraffin tissue samples of the investigated population (P<0.05(, but the number of positive samples for this virus is more in the blood samples, which indicates that the blood samples are more desire to trace this virus in patients.


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