The report of the latest achievement of molecular diagnosis and investigation of antibiotic resistance of Ureaplasma urealyticum and Neisseria gonorrhoeae isolated from cervical secretions of infertile women referring to the women's clinic of Shohadaye-Tajrish Educational-Treatment Center, Tehran

Document Type : Original Article

Authors

1 Associate Professor, Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Assistant Professor, Department of Medical Bacteriology, Men’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

3 Professor, Department of Obstetrics and Gynecology, Men’s Health and Reproductive Health Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

4 Associate Professor, Department of Biostatistics, Physiotherapy Research Center, School of Paramedicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract

Introduction: Neisseria and ureaplasma are considered to be the most important bacteria causing genital infection and infertility. The present study was conducted with the aim of searching for these two pathogens in the cervical secretions of infertile women, emphasizing molecular methods and determining drug resistance genes related to azithromycin and ciprofloxacin.
Methods: In this descriptive study, endocervical samples obtained from cervical secretions of 135 infertile women and 135 healthy women referred to Shohada Tajrish Hospital from October 2018 to September 2019 were examined. Data were analyzed by SPSS statistical software (version 21). Chi-square test was used to investigate the relationship between infection and infertility in individuals. P<0.05 was considered statistically significant.
Results: The prevalence of Neisseria gonorrhoea and Ureaplasma urealyticum was significant in the case group compared to the control group (P≤0.05). According to chi-square test, a significant relationship was observed between infection and infertility (P≤0.05). The prevalence of azithromycin resistance in Ureplasma urealyticum and Neisseria gonorrhoeae was 35 cases (48.6%) and 7 cases (58.3%), respectively. Ciprofloxacin resistance was reported in 3 cases (25%) of Neisseria gonorrhoeae and 64 cases (68.8%) of Ureaplasma urealyticum. The highest prevalence of resistance genes in both bacteria was related to mtrR and gyrA.
Conclusion: Antibiotic resistance in the studied bacteria indicates the indiscriminate use of antibiotics, the spread of antibiotic resistance gene structures, and genetic transmission among populations. In order to definitively treat and prevent the occurrence of resistance in pathogen strains, it is necessary to determine the resistance pattern to follow the resistance trend.

Keywords

References

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The Iranian Journal of Obstetrics, Gynecology and Infertility
Volume 25, Issue 12
February 2023
Pages 1-13

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