The effect of curcumin on rat ovarian reserve following ischemic reperfusion injury

Document Type : Original Article

Authors

1 Assistant professor, Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

2 M.Sc. of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

3 Professor, Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

4 Associate professor, Department of Social Medicine, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Abstract

Introduction: Ischemia-reperfusion injury is an important problem for ovarian torsion. It is generally caused by the damage of reactive oxygen species. Antioxidant agents such as curcumin may protect the ovaries from damage caused by oxygen free radicals. The present study was performed aimed to evaluate the effects of curcumin on ischemic-reperfusion (I / R) injury by evaluating biochemical and histopathological parameters.
Methods: Thirty-six female Wistar rats (200-250g) were divided into six experimental groups: control, sham, torsion (3 hours of ischemia), torsion and detour (3 hours of ischemia and 3 hours of reperfusion) and treatment groups 1 and 2 (Ischemia was divided into 3 hours, reperfusion for 3 hours and then intraperitoneal injection of 100 mg / kg curcumin half hour before detorsion and half hours after detorsion). Malondialdehyde (MDA) test was performed by taking blood samples from rats and special kits. After ovarian resection, hematoxylin and eosin (H&E) staining was performed for histopathological evaluation of the ovary. Data were analyzed by SPSS statistical software (version 18) and one-way variance and Kruskal-Willis statistical tests. P< 0.05 was considered statistically significant.
Results: The amount of malondialdehyde (MDA) showed a significant difference in the torsion and detour groups compared to other groups (P <0.001). In histopathological evaluation, the number of ovarian follicles at different stages of development showed a significant decrease in torsion and detour groups compared to control, sham and treatment groups (P <0.001), but no significant difference was observed between the treatment groups. The number of corpus luteum was significantly reduced in the treatment, torsion and detour groups compared to the control and sham groups (P <0.001).
Conclusion: Curcumin probably maintains ovarian function through its antioxidant and anti-inflammatory properties following ischemic-reperfusion injury.
Keywords: Curcumin, Ischemia, Ovary, Rat, Reperfusion.

Keywords

References

  1. Ali İP, Sinan TA, Aydın KU, Yeşilkaya Y, Orhan D. Prenatal over kisti torsiyonu: US ve MR görüntüleme bulguları. Journal of Clinical Obstetrics & Gynecology 2010; 20(4):262-5.
  2. Behroozi-Lak T, Ebrahimpour M, Zarei L, Pourjabali M, Farhad N, Mohaddesi H. Systemic administration of curcumin nanoparticles protects ischemia-reperfusion injury in ovaries: An animal model study. Revista da Associação Médica Brasileira 2018; 64:22-31.
  3. Ozkisacik S, Yazici M, Gursoy H, Culhaci N. Does gradual detorsion protect the ovary against ischemia–reperfusion injury in rats?. Pediatric surgery international 2014; 30(4):437-40.
  4. Celik O, Turkoz Y, Hascalik S, Hascalik M, Cigremis Y, Mizrak B, et al. The protective effect of caffeic acid phenethyl ester on ischemia-reperfusion injury in rat ovary. European Journal of Obstetrics & Gynecology and Reproductive Biology 2004; 117(2):183-8.
  5. Tawfik SS, Abouelella AM, Shahein YE. Curcumin protection activities against γ-rays-induced molecular and biochemical lesions. BMC Research Notes 2013; 6(1):1-10.
  6. González-Reyes S, Guzmán-Beltrán S, Medina-Campos ON, Pedraza-Chaverri J. Curcumin pretreatment induces Nrf2 and an antioxidant response and prevents hemin-induced toxicity in primary cultures of cerebellar granule neurons of rats. Oxidative medicine and cellular longevity 2013; 2013.
  7. Trujillo J, Chirino YI, Molina-Jijón E, Andérica-Romero AC, Tapia E, Pedraza-Chaverrí J. Renoprotective effect of the antioxidant curcumin: Recent findings. Redox biology 2013; 1(1):448-56.
  8. Aydin MS, Caliskan A, Kocarslan A, Kocarslan S, Yildiz A, Günay S, et al. Intraperitoneal curcumin decreased lung, renal and heart injury in abdominal aorta ischemia/reperfusion model in rat. International Journal of Surgery 2014; 12(6):601-5.
  9. Liu L, Zhang W, Wang L, Li Y, Tan B, Lu X, et al. Curcumin prevents cerebral ischemia reperfusion injury via increase of mitochondrial biogenesis. Neurochemical research 2014; 39(7):1322-31.
  10. Sak ME, Soydinc HE, Sak S, Evsen MS, Alabalik U, Akdemir F, et al. The protective effect of curcumin on ischemia-reperfusion injury in rat ovary. International Journal of Surgery 2013; 11(9):967-70.
  11. Baluchnejadmojarad T, Roghani M. Coenzyme q10 ameliorates neurodegeneration, mossy fiber sprouting, and oxidative stress in intrahippocampal kainate model of temporal lobe epilepsy in rat. Journal of Molecular Neuroscience 2013; 49(1):194-201.
  12. Mandarim-de-Lacerda CA. Stereological tools in biomedical research. Anais da Academia brasileira de Ciências 2003; 75(4):469-86.
  13. Kara M, Daglioglu YK, Kuyucu Y, Tuli A, Tap O. The effect of edaravone on ischemia–reperfusion injury in rat ovary. European Journal of Obstetrics & Gynecology and Reproductive Biology 2012; 162(2):197-202.
  14. Akgür FM, Kilinç K, Aktuĝ T. Reperfusion injury after detorsion of unilateral testicular torsion. Urological research 1993; 21(6):395-9.
  15. Vural H, Aksoy N, Ozbilge H. Alterations of oxidative–antioxidative status in human cutaneous leishmaniasis. Cell Biochemistry and Function: Cellular biochemistry and its modulation by active agents or disease 2004; 22(3):153-6.
  16. Carden DL, Granger DN. Pathophysiology of ischaemia–reperfusion injury. The Journal of pathology 2000; 190(3):255-66.
  17. Ergun Y, Koc A, Dolapcioglu K, Akaydin Y, Dogruer G, Kontas T, et al. The protective effect of erythropoietin and dimethylsulfoxide on ischemia-reperfusion injury in rat ovary. European Journal of Obstetrics & Gynecology and Reproductive Biology. 2010; 152(2):186-90.
  18. Kase N. The normal human ovary part I: Reproductive and endocrine functions. Altchek's Diagnosis and Management of Ovarian Disorders 2013: 1.
  19. Edgerton C, Crispín JC, Moratz CM, Bettelli E, Oukka M, Simovic M, et al. IL-17 producing CD4+ T cells mediate accelerated ischemia/reperfusion-induced injury in autoimmunity-prone mice. Clinical immunology 2009; 130(3):313-21.
  20. Menger MD, Rücker M, Vollmar B. Capillary dysfunction in striated muscle ischemia/reperfusion: on the mechanisms of capillary" no-reflow". Shock (Augusta, Ga.) 1997; 8(1):2-7.
  21. Halici Z, Karaca M, Keles ON, Borekci B, Odabasoglu F, Suleyman H, et al. Protective effects of amlodipine on ischemia-reperfusion injury of rat ovary: biochemical and histopathologic evaluation. Fertility and sterility 2008; 90(6):2408-15.
  22. Broncel M, Koter-Michalak M, Chojnowska-Jezierska J. The effect of statins on lipids peroxidation and activities of antioxidants enzymes in patients with dyslipidemia. Przeglad lekarski 2006; 63(9):738-42.
  23. Corbin JD, Francis SH. Pharmacology of phosphodiesterase-5 inhibitors. International journal of clinical practice 2002; 56(6):453-9.
  24. Fiscus RR. Involvement of cyclic GMP and protein kinase G in the regulation of apoptosis and survival in neural cells. Neurosignals 2002; 11(4):175-90.
  25. Javadi-Afshar A, Najafpour A. Effect of Systemic Administration of Curcumin on Ischemia-Reperfusion Injury in Ovaries: An Animal Model Study. Iranian Journal of Veterinary Surgery 2019; 14(1):1-8.
  26. Aggarwal BB, Harikumar KB. Potential therapeutic effects of curcumin, the anti-inflammatory agent, against neurodegenerative, cardiovascular, pulmonary, metabolic, autoimmune and neoplastic diseases. The international journal of biochemistry & cell biology 2009; 41(1):40-59.
  27. Srivastava G, Mehta JL. Currying the heart: curcumin and cardioprotection. Journal of cardiovascular pharmacology and therapeutics 2009; 14(1):22-7.
  28. Chen KH, Chao D, Liu CF, Chen CF, Wang D. Curcumin attenuates airway hyperreactivity induced by ischemia-reperfusion of the pancreas in rats. InTransplantation proceedings 2010; 42(3):744-7.
  29. Avci G, Kadioglu H, Sehirli AO, Bozkurt S, Guclu O, Arslan E, et al. Curcumin protects against ischemia/reperfusion injury in rat skeletal muscle. Journal of Surgical Research 2012; 172(1):e39-46.
The Iranian Journal of Obstetrics, Gynecology and Infertility
Volume 25, Issue 7
September 2022
Pages 14-21

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