Investigation the Association between Factor XIII Gene Polymorphism G103T with Recurrent Miscarriage in Iranian Patients

Document Type : Original Article

Authors

1 M.Sc. Student of Biology, Sciences and Research Branch, Islamic Azad University, Tehran, Iran.

2 Assistant Professor, Department of Medical Genetics, School of Medicine, Tarbiat Modares University, Tehran, Iran.

3 Assistant Professor, Department of Biology, Pishva Unit, Islamic Azad University, Varamin, Iran.

Abstract

Introduction: Recurrent miscarriage is a reproduction problem, experienced by 5% of couples. Considering the relationship between thrombophilia and abortion, the aim of this study was evaluating the relationship between factor XIII gene polymorphism G103T as a factor in the coagulation pathway and recurrent miscarriage.
Methods: This case control study was done during 2010-2011. Patients were divided into two groups; 105 women with 2 or more miscarriage as case group and 98 healthy women with two or more children and no history of abortion, as controls. DNA was extracted from their leukocytes using salting out method. The status of the G103T variant was determined by PCR-RFLP. Amplifying the corresponding region of genomic DNA followed by treatment with restriction enzyme Dde1. Data were analyzed using SPSS software version 18 and chi-square test. P value less than 0.05 was considered significant.
Results: 84 women (80%) in case group and 31 women (32%) in control group have 103GT/103TT genotypes and the difference was statistically significant (p<0.001). The allelic frequency for G103T variant also showed significant difference between two groups (p<0.001).
Conclusion: G103T polymorphism can cause miscarriage via coagulation system dysfunction.
 

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