Identification of Human Papillomavirus Type 16 among Thinprep Samples from 11 Provinces of Iran

Document Type : Original Article

Authors

1 M.Sc. Student of Medical Virology, Razi Vaccine and Serum Research Institute of Karaj, Karaj, Iran.

2 Ph.D. of Medical Virology, Razi Vaccine and Serum Research Institute of Karaj, Karaj, Iran.

3 B.Sc. of Laboratory Sciences, Virology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

4 M.Sc. of Medical Virology, Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

5 Associate Professor, Department of Medical Virology, Virology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract

Introduction: The importance of human papillomavirus (HPV) in developing of cervical cancer has been proved. Among HPVs, human papillomavirus type 16 (HPV-16) is accounted for in the first place in cervical cancer. So screening the human papillomavirus type 16 particularly is important. This study investigated the presence of HPV-16 in the ThinPrep samples collected from 11 provinces of Iran.
Methods: This cross sectional study was conducted on 108 ThinPrep samples which were included HPV DNA. The DNA of samples was extracted by Invitek kit. Then HPV-16 genome was sought using primers corresponding to the parts of the E6/E7 genome of HPV-16 by Nested PCR method. Data were analyzed using SPSS software version 13.
Results: 25% of samples (27 out of 108) were positive for HPV-16. Kerman province had the most rate of HPV-16 positive with having four HPV DNA positive samples and Qazvin province had the lowest rate of HPV-16 positive with having seven HPV DNA positive.
Conclusion: HPV-16 is the most important and prevalent type of HPV among Iranian woman and identification of the other high-risk HPV types is highly recommended.
 

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