Effect of combined exercises (aerobic-pilates) on the serum level of interleukin 10 and blood platelets in women with breast cancer

Document Type : Original Article


1 Associate Professor, Department of Exercise Sciences, School of Education Sciences and Psychology, Azarbaijan Shahid Madani University, Tabriz, Iran.

2 Associate Professor, Department of Exercise Sciences, School of Medical Science, Tabriz University of Medical Science, Tabriz, Iran.

3 M.Sc. of Exercise Physiology, School of Education Sciences and Psychology, Azarbaijan Shahid Madani University, Tabriz, Iran.

4 Ph.D. Student of Exercise Physiology, School of Education Sciences and Psychology, Tabriz University of Medical Science, Tabriz, Iran.


Introduction:Exercise can play a supportive role in the improvement of breast cancer by regulating and modulating cytokines. Interleukin 10 is an anti-inflammatory and anti-tumor cytokine that plays an important role in inhibiting tumor growth. This study was performed with aim to evaluate the effect of 12 weeks of combined exercise (aerobic_pilates) on the serum level of interleukin 10 and blood platelets in women with breast cancer.
Methods: This experimental study was performed on 30 women with breast cancer who had undergone at least 6 months of surgery and had received chemotherapy and were under treatment with medication in Azarbaijan Shahid Madani University in 2019. The subjects were divided into experimental and control groups. The experimental group performed 3 sessions of exercise each week (aerobic-pilates) for 12 weeks. During this time, the control group did not participate in any exercise or physical activity program. Blood samples were obtained before and 48 hours after exercise intervention to extract the variables. Data were analyzed by SPSS software (version 20) and independent and correlated t-test. P<0.05 was considered statistically significant.
Results: The results of correlated t-test showed that the serum level of interleukin 10 (P =0.001) and the number of blood platelets (P =0.041) significantly increased in the experimental group after 12 weeks of combined training (aerobic-Pilates) compared to the pre-test, while this change was not significant in the control group (p> 0.05).
Conclusion: Performing combined exercises (aerobic_Pilates) by improving the status of interleukin 10 as an anti-inflammatory and anti-tumor cytokine can be useful in improving the health status of women with breast cancer. Therefore, designing and performing such exercises in this population can solve many problems of these loved ones.


  1. Mousavi SM, Montazeri A, Mohagheghi MA, Jarrahi AM, Harirchi I, Najafi M, et al. Breast cancer in Iran: an epidemiological review. The breast journal 2007; 13(4):383-91.
  2. Abbastabar H, Soleymani DM, HamidiI Fard P, Jalilian F, Mirzaee Alvijeh M, Nasir Zadeh M. The relationship between breast cancer and the most common noncontagious disease risk factors: an ecologic study. Health System Research 2012; 8(3):369-76.
  3. Carpi A, Nicolini A, Antonelli A, Ferrari P, Rossi G. Cytokines in the management of high risk or advanced breast cancer: an update and expectation. Curr Cancer Drug Targets 2009; 9(8):888-903.
  4. Apryani E, Ali U, Wang ZY, Wu HY, Mao XF, Ahmad KA, et al. The spinal microglial IL-10/β-endorphin pathway accounts for cinobufagin-induced mechanical antiallodynia in bone cancer pain following activation of α7-nicotinic acetylcholine receptors. J Neuroinflammation 2020; 17(1):75.
  5. Mocellin S, Marincola FM, Young HA. Interleukin-10 and the immune response against cancer: a counterpoint. J Leukoc Biol 2005; 78(5):1043-51.
  6. Kundu N, Beaty TL, Jackson MJ, Fulton AM. Antimetastatic and antitumor activities of interleukin 10 in a murine model of breast cancer. J Natl Cancer Inst 1996; 88(8):536-41.
  7. Pinzon‐Charry A, Maxwell T, López JA. Dendritic cell dysfunction in cancer: a mechanism for immunosuppression. Immunology and cell biology 2005; 83(5):451-61.
  8. Taga K, Cherney B, Tosato G. IL-10 inhibits apoptotic cell death in human T cells starved of IL-2. International immunology 1993; 5(12):1599-608.
  9. Kushi LH, Doyle C, McCullough M, Rock CL, Demark-Wahnefried W, Bandera EV, et al. American Cancer Society Guidelines on nutrition and physical activity for cancer prevention: reducing the risk of cancer with healthy food choices and physical activity. CA Cancer J Clin 2012; 62(1):30-67.
  10. Jones LW, Viglianti BL, Tashjian JA, Kothadia SM, Keir ST, Freedland SJ, et al. Effect of aerobic exercise on tumor physiology in an animal model of human breast cancer. J Appl Physiol (1985) 2010; 108(2):343-8.
  11. Murphy EA, Davis JM, Barrilleaux TL, McClellan JL, Steiner JL, Carmichael MD, et al. Benefits of exercise training on breast cancer progression and inflammation in C3(1)SV40Tag mice. Cytokine 2011; 55(2):274-9.
  12. Radmehr L, Kalantari Khandani B, KAZEMI A. The Effect of 8 weeks of endurance training on serum levels of IL-10 and IL-17 in elderly women with breast cancer. Daneshvar Medicine 2016; 23(122):39-46.
  13. Shiri Y, Agha Alinejad H, Gharakhanlou R, Amani Shalamzari S, Saei MA. Effect of six weeks endurance training on tumor tissue IL-10 cytokine levels in breast cancer bearing mice. Iranian Journal of Endocrinology and Metabolism 2014; 16(3):205-210.
  14. Fathi Bayatiani Z, Debidi Roshan V, Rudbari F. Effects of Interleukin 10 Cardiac Protection Protocols and Respiratory Cardiac Indicators in Obese Women with Breast Cancer. Iranian Journal of Diabetes and Metabolism 2012; 12(1):68-81.
  15. Maghsoudi N, Khosravi N, Ravasi A. The effect of selected training (aerobics and strength) on some cytokines in male and female patients with MS 2011: 5-23.
  16. Faraji K, Neamatzade S, Saboori H. Radiation effect on the number of circulating blood cells in patients receiving radiation therapy in Tohid hospital of Sanandaj in 2014. Zanko J Med Sci 2014; 15(46):49-58
  17. El-Sayed MS, Ali ZE, Ahmadizad S. Exercise and training effects on blood haemostasis in health and disease. Sports medicine 2004; 34(3):181-200.
  18. Ajam M, Aghayari A, Salek R, Haghverdian S, Gheitasi M. Effect of 12 weeks Aerobic Exercise Training on Some Characteristics of Immune System and General Health of Women with Breast Cancer. JSMT 2014; 12(7):41-54
  19. American College of Sports Medicine, editor. ACSM's health-related physical fitness assessment manual. Lippincott Williams & Wilkins; 2013.
  20. Donatto FF, Neves RX, Rosa FO, Camargo RG, Ribeiro H, Matos-Neto EM, et al. Resistance exercise modulates lipid plasma profile and cytokine content in the adipose tissue of tumour-bearing rats. Cytokine 2013; 61(2):426-32.
  21. Kizaki T, Takemasa T, Sakurai T, Izawa T, Hanawa T, Kamiya S, et al. Adaptation of macrophages to exercise training improves innate immunity. Biochemical and biophysical research communications 2008; 372(1):152-6.
  22. Vear NK, Coombes JS, Bailey TG, Skinner TL. The Interplay between Vascular Function and Sexual Health in Prostate Cancer: The Potential Benefits of Exercise Training. Medical Sciences 2020; 8(1):11.
  23. Posthuma JJ, van der Meijden PE, ten Cate H, Spronk HM. Short-and Long-term exercise induced alterations in haemostasis: a review of the literature. Blood Reviews 2015; 29(3):171-8.
  24. Hügli S, Hügli A, Simonetta F, Chalandon Y. A rare case of immune thrombocytopenia secondary to breast cancer. Clinical Case Reports 2019; 7(1):170.
  25. Seegobin K, Gharia B, Maharaj S, Zuberi L. Coexistent Breast Cancer and Essential Thrombocythemia: How We Addressed the Therapeutic Challenges. Case reports in hematology 2018; 2018.
  26. Lal I, Dittus K, Holmes CE. Platelets, coagulation and fibrinolysis in breast cancer progression. Breast Cancer Research 2013; 15(4):1-11.
  27. Koelwyn GJ, Quail DF, Zhang X, White RM, Jones LW. Exercise-dependent regulation of the tumour microenvironment. Nature Reviews Cancer 2017; 17(10):620-32.