Relationship between human papillomavirus and breast cancer in women with this cancer in Khuzestan province

Document Type : Original Article


1 M.Sc. student of Biochemistry, School of Sciences, Lorestan University, Khorramabad, Iran.

2 Assistant Professor, Department of Biology, School of Sciences, Lorestan University, Khorramabad, Iran.


Introduction: Breast cancer is one of the most common malignancies in the world. Although the etiology of breast cancer is not completely known, but exposure to Human papilloma virus (HPV) is suggested as a risk factor for breast cancer. This study was performed with aim to investigate the presence of HPV-DNA in tissue samples of healthy and breast cancer women in Khuzestan province.
Methods: In this retrospective study conducted in 2018, 40 paraffin blocks were collected from patients with diagnosis of breast malignancy and 20 healthy paraffin blocks from those without malignant lesion (Fibro adenoma) in Khuzestan province. After DNA extraction, amplification of beta-actin housekeeping gene was done for evaluation of DNA purity. The presence of DNA-HPV16,18 was examined by polymerase chain reaction (PCR) using virus specific primers. Data were analyzed by SPSS software (version 16) and Cramer test. P<0.05 was considered statistically significant.
Results: DNA-HPV16 was detected in 25 cases of 40 breast cancer samples (%62.5), and DNA-HPV 18 was detected in 10 cases of 40 breast cancer samples (%25). None of the 20 control samples showed the presence of DNA-HPV16 and DNA-HPV18. Statistically, the Cramer index for HPV16 and HPV18 infection in cancer sample was calculated 0.598 and 0.316, respectively.
Conclusion: There is a significant relationship between breast cancer and HPV16,18 virus infection in women of Khuzestan province.


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