ارتباط پاپیلوما ویروس انسانی و سرطان پستان در زنان مبتلا به این سرطان در استان خوزستان

نوع مقاله : اصیل پژوهشی

نویسندگان

1 دانشجوی کارشناسی ارشد بیوشیمی، دانشکده علوم، دانشگاه لرستان، خرم‌آباد، ایران.

2 استادیار گروه زیست‌شناسی، دانشکده علوم، دانشگاه لرستان، خرم‌آباد، ایران.

چکیده

مقدمه: سرطان پستان یکی از شایع‌ترین بدخیمی در جهان است، اگرچه علت سرطان پستان به‌طور کامل شناخته نشده است، اما قرار گرفتن در معرض ویروس پاپیلوما انسانی (HPV) به‌عنوان یک عامل خطر برای سرطان پستان پیشنهاد می‌شود. مطالعه حاضر با هدف بررسی حضورHPV-DNA در نمونه‌های بافتی سرطان پستان و سالم در زنان استان خوزستان انجام شد.
روش‌کار: در این مطالعه گذشته‌نگر که در سال 1397 انجام شد،40 بافت پارافینه سرطان از بیماران با تشخیص بدخیمی پستان و 20 بافت پارافینه پستان از افراد بدون ضایعه توموری بدخیم (فیبرو آدنوما) از زنان استان خوزستان جمع­آوری شدند. پس از استخراج DNA، تکثیر با ژن خانه­دار بتااکتین به‌منظور بررسی کیفیت DNA استخراج‌ شده انجام گرفت. حضور DNA ویروس HPV16,18 با روش PCR و استفاده از آغازگرهای اختصاصی ویروس مورد بررسی قرار گرفت. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS (نسخه 16) و آزمون کرامر انجام شد. میزان p کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته­ها: DNA ویروس HPV16 در 25 مورد (5/62%) از 40 نمونه توموری و DNA ویروس HPV18 در 10 مورد (25%) از 40 نمونه توموری مشاهده شد. هیچ‌یک از نمونه­های شاهد حضور DNA ویروس HPV16 و HPV18 را نشان نداد. از لحاظ آماری ضریب کرامر برای ویروس HPV16 و HPV18 در نمونه‌های سرطانی به‌ترتیب برابر با 598/0 و 316/0 محاسبه گردید.
نتیجه­گیری: بین سرطان پستان و عفونت ویروس‌های HPV16,18 در زنان استان خوزستان ارتباط معناداری وجود دارد.

کلیدواژه‌ها


عنوان مقاله [English]

Relationship between human papillomavirus and breast cancer in women with this cancer in Khuzestan province

نویسندگان [English]

  • Pegah Hosseinpouri 1
  • Seyed Hesamaldin Hejazi 2
  • Faranak Hadi 2
1 M.Sc. student of Biochemistry, School of Sciences, Lorestan University, Khorramabad, Iran.
2 Assistant Professor, Department of Biology, School of Sciences, Lorestan University, Khorramabad, Iran.
چکیده [English]

Introduction: Breast cancer is one of the most common malignancies in the world. Although the etiology of breast cancer is not completely known, but exposure to Human papilloma virus (HPV) is suggested as a risk factor for breast cancer. This study was performed with aim to investigate the presence of HPV-DNA in tissue samples of healthy and breast cancer women in Khuzestan province.
Methods: In this retrospective study conducted in 2018, 40 paraffin blocks were collected from patients with diagnosis of breast malignancy and 20 healthy paraffin blocks from those without malignant lesion (Fibro adenoma) in Khuzestan province. After DNA extraction, amplification of beta-actin housekeeping gene was done for evaluation of DNA purity. The presence of DNA-HPV16,18 was examined by polymerase chain reaction (PCR) using virus specific primers. Data were analyzed by SPSS software (version 16) and Cramer test. P<0.05 was considered statistically significant.
Results: DNA-HPV16 was detected in 25 cases of 40 breast cancer samples (%62.5), and DNA-HPV 18 was detected in 10 cases of 40 breast cancer samples (%25). None of the 20 control samples showed the presence of DNA-HPV16 and DNA-HPV18. Statistically, the Cramer index for HPV16 and HPV18 infection in cancer sample was calculated 0.598 and 0.316, respectively.
Conclusion: There is a significant relationship between breast cancer and HPV16,18 virus infection in women of Khuzestan province.

کلیدواژه‌ها [English]

  • Breast Cancer
  • Human papilloma virus
  • PCR
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