The Iranian Journal of Obstetrics, Gynecology and Infertility

The Iranian Journal of Obstetrics, Gynecology and Infertility

Za0Using the PCR method for detection of bacterial vaginosis in women suspected of vaginosis in Guilan province

Document Type : Original Article

Authors
Esmaeil Roohbakhsh 1
Ali Mojtahedi 2
Ramazan Ali Khavari-Nezhad 3
Nour Amirmozafari 4
1 PhD Candidate, Department of Microbiology, School of Sciences, Tehran University of Research Sciences, Tehran, Iran.
2 Associate Professor, Department of Bacteriology, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran.
3 Professor, Department of Biology, School of Sciences, Tehran University of Research Sciences, Tehran, Iran.
4 Professor, Department of Bacteriology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
10.22038/ijogi.2019.12655
Abstract
Introduction: Bacterial vaginosis is the most common lower genital tract infection among women in reproductive age. Its causative agent is often the bacterium Gardnerella vaginalis which exists in synergism with other mostly anaerobic bacteria such as Atopobium vaginae, Mobiluncus curtisii and Megasphaera type I. The present study was conducted with aim of measuring molecular measurements (PCR) in the detection and diagnosis of bacterial vaginosis in women.
Methods: This descriptive study was performed on 100 women referred to Obstetrics and Gynecology Clinics of Al-Zahra Hospital and private clinics in Rasht from September 2016 to October 2017. They were examined and tested for bacterial vaginosis. The presence of Gardnerella vaginalis was tested by five different laboratory methods based on Amsel criteria and molecular methods including determination of appearance characteristics, determination of pH, Whiff test, and observation of Clue cells in direct smear, conducting Polymerase Chain Reaction (PCR) using specific primers on DNA extracted from vaginal specimens. Data were analyzed using SPSS software (version 21) and Chi-square test. P<0.05 was considered significant.
Results: In this study, from a total of 100 suspected women who were examined for vaginal infection with use of Amsel method, 31 cases (31%) of vaginosis were confirmed. Using the molecular method of PCR in women with vaginosis (Amsel method), Gardnerella vaginosis was confirmed in 14 (45%) and Atopobioum vagine in 10 (32%).
Conclusion: Using the PCR method showed that Gardenerella vaginalis and Atopobium vaginae play an important role in womenaged 18-35-year-old with bacterialvaginosis that indicates the role of these two bacteria in the development of bacterial vaginosis.
Keywords:
Keywords
Atopobium vagine
Bacterial vaginosis
Gardnerella vaginalis
PCR
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The Iranian Journal of Obstetrics, Gynecology and Infertility
Volume 21, Issue 12
March 2019
Pages 29-36