نوع مقاله : اصیل پژوهشی
نویسندگان
1 گروه بیولوژی و آناتومی دانشکده پزشکی دانشگاه علوم پزشکی شهید صدوقی - یزد- ایران
2 گروه بیولوژی و آناتومی دانشکده پزشکی دانشگاه شهید صدوقی یزد
3 گروه بیولوژی و آناتومی دانشکده پزشکی دانشگاه علو پزشکی شهید صدوقی یزد
4 گروه بیولوژی و علوم تشریح، دانشکده پزشکی، دانشگاه علوم پزشکی شهید صدوقی ، یزد، ایران
5 گروه پزشکی اجتماعی، دانشکده پزشکی، دانشگاه علوم پزشکی شهید صدوقی، یزد، ایران.
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Introduction: The aim of this study was to evaluate the effects of curcumin on ischemic-reperfusion (I / R) injury by evaluating biochemical and histopathological parameters.
Materials and Methods: Thirty-six female Wistar rats (200-250g) were divided into six experimental groups: control, sham, torsion (3 hours of ischemia), torsion and detour (3 hours of ischemia and 3 hours of reperfusion) and treatment groups 1 and 2 (to Ischemia was divided into 3 hours, reperfusion for 3 hours and then intraperitoneal injection of 100 mg / kg curcumin (0.5 hours before detorsion and 0.5 hours after detorsion). Malondialdehyde (MDA) test was performed by taking blood samples from rats and special kits. After ovarian resection, hematoxylin and eosin (H&E) staining was performed for histopathological evaluation of the ovary.
Results: The amount of malondialdehyde (MDA) in the torsion and detour groups showed a significant difference compared to other groups (P <0.001). In histopathological evaluation, the number of ovarian follicles at different stages of development in torsion and detour groups showed a significant decrease compared to control, sham and treatment groups (P <0.001), but no significant difference was observed between treatment groups. The number of corpus luteum in the treatment, torsion and detour groups was significantly reduced compared to the control and sham groups (P <0.001).
Conclusion: The results of this study showed that curcumin may reduce ischemic-reperfusion damage of the ovary through its antioxidant and anti-inflammatory properties.
کلیدواژهها [English]