بررسی ارتباط میزان آلفافتوپروتئین، آلکالن فسفاتاز و فریتین مایع آمنیوتیک سه ماهه دوم حاملگی با زایمان

نوع مقاله: اصیل پژوهشی

نویسندگان

1 استادیار گروه پریناتولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان، زنجان، ایران.

2 دکترای تخصصی زنان و مامایی، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان، زنجان، ایران.

3 استاد گروه آمار زیستی، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان، زنجان، ایران.

4 دانشیار گروه ایمنی‌شناسی پزشکی، مرکز تحقیقات ژن‌درمانی سرطان، دانشکده پزشکی، دانشگاه علوم پزشکی زنجان، زنجان، ایران.

5 مربی گروه مامایی، دانشکده پرستاری و مامایی، دانشگاه علوم پزشکی زنجان، زنجان، ایران.

چکیده

مقدمه: زایمان زودرس، یکی از عارضه‌های مهم بارداری است که تأثیر بسزایی در سلامت و مرگ‌و‌میر نوزادان دارد، لذا پیش­بینی و تشخیص زودهنگام آن از اهمیت بسیار زیادی برخوردار است. مطالعه حاضر با هدف بررسی ارتباط میزان آلفافتوپروتئین، آلکالن فسفاتاز و فریتین مایع آمنیوتیک سه ماهه دوم حاملگی و زایمان پره­ترم انجام شد.
روش‌کار: این مطالعه مقطعی تحلیلی در بازه زمانی شهریور سال 1393 تا بهمن ماه 1394 بر روی 60 نفر از افرادی که به‌دلیل غربالگری مثبت سه ماهه اول، بین هفته‌های 20-15 (میانگین 17 هفته) آمنیوسنتز شده بودند؛ انجام شد. میزان آلفافتوپروتئین، آلکالن فسفاتاز و فرتین نمونه‌های فریز شده 40 نفر با زایمان ترم و 20 نفر با زایمان پره‌ترم با روش اسپکتروفتومتری و الایزا مورد آزمایش قرار گرفت. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری SPSS (نسخه 22) و آزمون‌های تی و کای دو انجام گرفت. میزان p کمتر از 05/0 معنی‌دار در نظر گرفته شد.
یافته‌ها: میانگین میزان آلفافتوپروتئین در زایمان ترم 44/96±14/382 و در زایمان پره‌ترم 83/122±47/547 نانوگرم بر میلی‌لیتر، میزان آلکالن فسفاتاز در زنان ترم 01/13±35/27 و در زنان پره‌ترم 51/15±45/74 یونیت بر لیتر و میزان فریتین زایمان ترم 44/96±14/382 و در زایمان پره‌ترم 83/122±47/547 نانوگرم بر میلی‌لیتر بود کهمیزان هر سه نشانگر در زایمان پره‌ترم نسبت به زایمان ترم به‌طور معناداری بالاتر بود (001/0>p).
نتیجه‌گیری: بین میزان آلفافتوپروتئین، آلکالن فسفاتاز و فریتین مایع آمنیون و زایمان پره­ترم، ارتباط مثبت و معنی‌داری وجود دارد که ممکن است به درک ما از پاتوفیزیولوژی زایمان زودرس کمک کند. با توجه به نتایج به‌‌دست آمده توصیه می‌شود تحقیقات دیگری با تعداد نمونه بیشتر نیز صورت گیرد.

کلیدواژه‌ها


عنوان مقاله [English]

The relationship between amniotic fluid alpha-fetoprotein, alkaline phosphatase and ferritin in the second trimester of pregnancy and preterm birth

نویسندگان [English]

  • Atousa Dabiri Oskoei 1
  • Mahsa Jamshidasl 2
  • Soghrat Faghihzadeh 3
  • Abdolreza Esmaeilzadeh 4
  • Fatemeh Bayat 5
1 Assistant Professor, Department of Perinatology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
2 Gynecologist, Department of Obstetrics and Gynecology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
3 Professor, Department of Biostatistics, Faculty of Medicine, Zanjan University of Medical Science, Zanjan, Iran.
4 Associate Professor, Department of Medical Immunology, Cancer Gene Therapy Research Center, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
5 Instructor, Department of Midwifery, School of Nursing and Midwifery, Zanjan University of Medical Science, Zanjan, Iran.
چکیده [English]

Introduction: Preterm birth is one of the most important complications of pregnancy, which has a significant impact on the health and mortality of neonates, so early diagnosis and prediction is vital. This study was performed with aim to investigate the relationship between amniotic fluid alpha-fetoprotein (αFP), alkaline phosphatase (ALP) and ferritin in the second trimester of pregnancy and preterm birth. 
Methods: This cross-sectional analytical study was performed on 60 subjects with gestational age of 15-20 weeks (mean of 17 weeks) who had performed amniocentesis due to positive first-trimester screening from September 2014 to February 2015. The levels of αFP, ALP and ferritin were tested using spectrophotometric and ELISA method in freeze samples of 40 subjects with term birth and 20 subjects with preterm birth. Data were analyzed by SPSS software (version 22) and t-test and Chi-square test. P<0.05 was considered statistically significant.
Results: The mean amniotic fluid αFP in term and preterm birth was 382.14 ± 96.44 and 547.47 ± 122.83(ng/ml), respectively; the mean ALP in term and preterm birth was 27.35 ± 13.01 and 74.45±15.51 (IU/ L), respectively; and the mean ferritin was 382.14 ± 96.44, and 547.47±122.83 (ng/ml), respectively. The level of all three markers was significantly higher in preterm birth than term birth (p < 0.001).
Conclusion: There is significant and positive relationship between amniotic fluid αFP, ALP and ferritin levels with preterm birth that may help to increase our understanding of the pathophysiology of preterm birth. According to the results, it is recommended to perform further research with larger sample sizes.

کلیدواژه‌ها [English]

  • Alkaline phosphatase
  • alpha-Fetoproteins
  • Amniocentesis
  • Ferritins
  • Preterm Birth
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