مطالعه سطح سرمی Angiopoietin-like protein 4 در زنان مبتلا به سندرم تخمدان پلی‌کیستیک با توده بدنی نرمال

نوع مقاله: اصیل پژوهشی

نویسندگان

1 دانشیار گروه زنان و مامایی، دانشکده پزشکی، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران.

2 دانشیار گروه بیوشیمی بالینی، دانشکده پزشکی، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران.

3 دانشجوی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران.

4 دانشیار گروه پزشکی اجتماعی، دانشکده پزشکی، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران.

5 کارشناس علوم آزمایشگاهی، مرکز تحقیقات طب گرمسیری خلیج فارس، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران.

6 دانشجوی کارشناسی ارشد بیوشیمی، دانشکده پزشکی، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران.

7 کارشناس ارشد فیزیولوژی پزشکی، مرکز تحقیقات طب گرمسیری خلیج فارس، دانشگاه علوم پزشکی بوشهر، بوشهر، ایران.

چکیده

مقدمه: سندرم تخمدان پلی‌کیستیک (PCOS) یک بیماری اندوکرین پیچیده در زنان می‌باشد و تقریباً 75% از زنان PCOS دچار افزایش وزن هستند. Angiopoietin-like protein 4 (ANGPTL4) مهار‌کننده قوی آنزیم لیپوپروتئین ‌لیپاز (LPL) می‌باشد. این پروتئین تعیین‌کننده غلظت و کلیرانس تری‌گلیسرید ‌پلاسما می‌باشد. مطالعه حاضر با هدف بررسی سطح سرمی ANGPTL4 در زنان PCOS با شاخص توده بدنی نرمال انجام شد.
روش‌‌کار: این مطالعه مورد- شاهدی در سال 95-1394 بر روی 47 زن مراجعه کننده به درمانگاه ابوالفضل وابسته به دانشگاه علوم پزشکی بوشهر انجام شد. در این مطالعه 25 زن  PCOSبه روش غیر‌احتمالی آسان و بر اساس معیار‌های تشخیصی روتردام (2003) به عنوان گروه مورد و 22 زن سالم به عنوان گروه شاهد انتخاب شدند. سطح سرمی تمام هورمون‌ها به کمک تکنیک الایزا و پارامترهای بیوشیمیایی نیز به کمک اتوآنالیزر اندازه‌گیری شد. تجزیه و تحلیل داده‌ها با استفاده از نرم‌افزار آماری  SPSS(نسخه 16) و آزمون‌های تی مستقل، من ویتنی و رگرسیون خطی انجام شد. میزان p‌ کمتر از 05/0 معنادار در نظر گرفته شد.
یافته‌ها: نتایج این مطالعه افزایش معناداری در میزان LH (003/0=p)،  LH/FSH(007/0=p)، FBS (03/0=p)، تستوسترون (02/0=p) و پرولاکتین (005/0=p) در گروه مورد نشان داد. تفاوت معناداری در میزان ANGPTL4، انسولین، HOMA-IR، لیپوپروتئین‌های با چگالی پایین (LDL)، HDL، HOMA-B و کراتینین در گروه مورد نسبت به گروه شاهد مشاهده نشد. ارتباط معکوس و معناداری بین سطح سرمی ANGPTL4 با لگاریتم انسولین (002/0=p)، FBS (006/0=p)، لگاریتم HOMA-IR (001/0=p) و شاخص توده بدنی (03/0=p) وجود داشت، اما ارتباطی بین ANGPTL4 و پارامترهای لیپیدی مشاهده نشد.
نتیجه‌گیری: در مطالعه حاضر تفاوت معناداری در سطح سرمی ANGPTL4 در زنان مبتلا به تخمدان پلی‌کیستیک با توده بدنی نرمال در مقایسه با گروه شاهد مشاهده نشد.

کلیدواژه‌ها


عنوان مقاله [English]

Serum levels of angiopoietin-like protein 4 in polycystic ovary syndrome women with normal body mass index

نویسندگان [English]

  • Elham Rahmani 1
  • Samad Akbarzadeh 2
  • Abdollah Nazari 3
  • Niloofar Motamed 4
  • Soraya Ghodrati 5
  • Anahita Abasi Fard 6
  • Marzieh Zohrabi 7
1 Associate Professor, Department of Obstetrics and Gynecology, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
2 Associate Professor, Department of Clinical Biochemistry, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
3 Medical Student, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
4 Associate Professor, Department of Social Medicine, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
5 B.Sc. in Laboratory Sciences, Gulf Tropical Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.
6 M.Sc. Student of Clinical Biochemistry, Faculty of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran.
7 M.Sc. in Medical Physiology, Gulf Tropical Medicine Research Center, Bushehr University of Medical Sciences, Bushehr, Iran.
چکیده [English]

Introduction: Polycystic ovary syndrome (PCOS) is a complex endocrine disorder in women, and approximately 75% of women affected by PCOS are overweight. Angiopoietin-like protein 4 (ANGPTL4) is a potent inhibitor of lipoprotein lipase (LPL) enzyme. This protein is a determinant of plasma concentration and clearance of triglyceride. In this study, we aimed to evaluate the serum levels of ANGPTL4 in PCOS women with normal body mass index (BMI).
Methods: A case-control study of 47 women visiting Abolfazle Clinic, affiliated to Bushehr University of Medical Sciences, was performed during 2015-2016. In this study, 25 PCOS women were assigned to the case group through simple non-probable sampling based on the Rotterdam 2003 criteria. Further, 22 healthy women were selected for the control group. Serum levels of all hormones were measured by the ELISA technique, and biochemical parameters were evaluated by an autoanalyser. The obtained data were analyzed using independent samples t-test, Mann-Whitney U test, and linear regression test in SPSS, version 16. P-values less than 0.05 were considered statistically significant.
Results: The results of this study showed a significant increase in LH (P=0.003), LH/FSH (P= 0.007), FBS (P= 0.03), testosterone (P= 0.02), and prolactin (P= 0.005) levels in the case group. There was no significant difference in the levels of ANGPTL4, insulin, HOMA-IR, low-density lipoprotein (LDL), HDL, HOMA-B, and creatinine between the groups. There was a significant negative correlation between serum level of ANGPTL4 and insulin logarithm (P=0.002), FBS (P=0.006), HOMA-IR logarithm (P=0.001), and BMI (P=0.03), but no correlation was found between ANGPTL4 and lipid parameters.
Conclusion: We found no significant difference between the PCOS women with normal BMI and the controls regarding serum levels of ANGPTL4.

کلیدواژه‌ها [English]

  • Polycystic ovary syndrome (PCOS)
  • Angiopoietin-like protein 4 (ANGPTL4)
  • Lipoprotein lipase (LPL)
  • Normal BMI
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